• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码 RNA PCED1B-AS1 通过调控 miR-633/HOXA9 轴促进结直肠腺癌的增殖。

Long non-coding RNA PCED1B-AS1 promotes the proliferation of colorectal adenocarcinoma through regulating the miR-633/HOXA9 axis.

机构信息

Department of Gastrointestinal Surgery, Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou City, Jiangsu Province, PR, China.

出版信息

Bioengineered. 2022 Mar;13(3):5407-5420. doi: 10.1080/21655979.2022.2037225.

DOI:10.1080/21655979.2022.2037225
PMID:35176937
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8974004/
Abstract

Long non-coding RNA (lncRNA) PCED1B-AS1 was shown to play essential roles in human cancers, while its function in colorectal adenocarcinoma remains unclear. This study was carried out to investigate the function of PCED1B-AS1 in regulating the microRNA(miR)-633/HOXA9 axis in colorectal adenocarcinoma. The expression of PCED1B-AS1, miR-633 and HOXA9 was measured by quantitative real-time PCR (qRT-PCR) or Western blot analysis. Cell behaviors of colorectal adenocarcinoma cell lines were assessed by CCK-8, EdU, Transwell and flow cytometry assays. The interaction among PCED1B-AS1, miR-633 and HOXA9 was determined by luciferase reporter and RIP assays. Rescue experiments were performed to determine the regulatory axis in colorectal adenocarcinoma. Moreover, an animal model was established to verify the role of PCED1B-AS1. We found that PCED1B-AS1 was upregulated and miR-633 was downregulated in colorectal adenocarcinoma tissues and corresponding cell lines. Knockdown of PCED1B-AS1 inhibited cell proliferation and promoted apoptosis, while miR-633 inhibitor elevated proliferation and reduced apoptosis of cancer cell lines. In addition, overexpression of HOXA9 obviously attenuated the protective role of knockdown of PCED1B-AS1 or miR-633 mimics in colorectal adenocarcinoma progression. PCED1B-AS1 could negatively regulate the expression of HOXA9 by sponging miR-633. The experiments confirmed the role of PCED1B-AS1 and miR-633 in colorectal adenocarcinoma, as well as the regulatory relationship of this axis. Our results demonstrated that knockdown of PCED1B-AS1 inhibited the progression of colorectal adenocarcinoma by regulating the miR-633/HOXA9 axis.

摘要

长链非编码 RNA（lncRNA）PCED1B-AS1 被证明在人类癌症中发挥着重要作用，但其在结直肠腺癌中的作用尚不清楚。本研究旨在探讨 PCED1B-AS1 在调节结直肠腺癌细胞中 microRNA（miR）-633/HOXA9 轴中的作用。通过定量实时 PCR（qRT-PCR）或 Western blot 分析测量 PCED1B-AS1、miR-633 和 HOXA9 的表达。通过 CCK-8、EdU、Transwell 和流式细胞术测定结直肠腺癌细胞系的细胞行为。通过荧光素酶报告和 RIP 测定确定 PCED1B-AS1、miR-633 和 HOXA9 之间的相互作用。进行挽救实验以确定结直肠腺癌中的调节轴。此外，建立了动物模型以验证 PCED1B-AS1 的作用。我们发现 PCED1B-AS1 在结直肠腺癌组织和相应的细胞系中上调，而 miR-633 下调。PCED1B-AS1 的敲低抑制了细胞增殖并促进了细胞凋亡，而 miR-633 抑制剂则升高了癌细胞系的增殖并降低了凋亡。此外，HOXA9 的过表达明显减弱了敲低 PCED1B-AS1 或 miR-633 模拟物对结直肠腺癌进展的保护作用。PCED1B-AS1 可以通过海绵 miR-633 负调控 HOXA9 的表达。这些实验证实了 PCED1B-AS1 和 miR-633 在结直肠腺癌中的作用，以及该轴的调节关系。我们的结果表明，敲低 PCED1B-AS1 通过调节 miR-633/HOXA9 轴抑制结直肠腺癌的进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/630d3579a73f/KBIE_A_2037225_F0008_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/503bdb788882/KBIE_A_2037225_UF0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/f10193bf73c2/KBIE_A_2037225_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/600039816a60/KBIE_A_2037225_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/d127970d92d0/KBIE_A_2037225_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/aadd486f60c9/KBIE_A_2037225_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/50cf5d183a58/KBIE_A_2037225_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/630d3579a73f/KBIE_A_2037225_F0008_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/503bdb788882/KBIE_A_2037225_UF0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/f10193bf73c2/KBIE_A_2037225_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/600039816a60/KBIE_A_2037225_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/d127970d92d0/KBIE_A_2037225_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/aadd486f60c9/KBIE_A_2037225_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/50cf5d183a58/KBIE_A_2037225_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d05/8974004/630d3579a73f/KBIE_A_2037225_F0008_OC.jpg

相似文献

1
Long non-coding RNA PCED1B-AS1 promotes the proliferation of colorectal adenocarcinoma through regulating the miR-633/HOXA9 axis.长链非编码 RNA PCED1B-AS1 通过调控 miR-633/HOXA9 轴促进结直肠腺癌的增殖。
Bioengineered. 2022 Mar;13(3):5407-5420. doi: 10.1080/21655979.2022.2037225.
2
Long non-coding RNA PCED1B antisense RNA 1 promotes gastric cancer progression via modulating microRNA-215-3p / C-X-C motif chemokine receptor 1 axis.长非编码 RNA PCED1B 反义 RNA 1 通过调节 microRNA-215-3p/C-X-C 基序趋化因子受体 1 轴促进胃癌进展。
Bioengineered. 2021 Dec;12(1):6083-6095. doi: 10.1080/21655979.2021.1971503.
3
Long Non-coding RNA PCED1B Antisense RNA 1 Promotes Cell Proliferation and Invasion in Hepatocellular Carcinoma by Regulating the MicroRNA-34a/CD44 Axis.长链非编码 RNA PCED1B 反义 RNA 1 通过调控 microRNA-34a/CD44 轴促进肝癌细胞增殖和侵袭。
Curr Med Sci. 2024 Jun;44(3):503-511. doi: 10.1007/s11596-023-2823-5. Epub 2024 May 15.
4
LncRNA PCED1B-AS1 mediates miR-3681-3p/MAP2K7 axis to promote metastasis, invasion and EMT in gastric cancer.长链非编码RNA PCED1B-AS1通过介导miR-3681-3p/MAP2K7轴促进胃癌的转移、侵袭和上皮-间质转化。
Biol Direct. 2024 May 2;19(1):34. doi: 10.1186/s13062-024-00468-z.
5
Long Non-Coding RNA HAND2 Antisense RNA 1 Inhibits Colorectal Cancer Development by Sponging microRNA-3118/Leptin Receptor Axis.长链非编码 RNA HAND2 反义 RNA 1 通过海绵 microRNA-3118/瘦素受体轴抑制结直肠癌的发展。
Ann Clin Lab Sci. 2023 Jan;53(1):82-93.
6
Long Non-Coding RNA PCED1B-AS1 Promotes the Progression of Clear Cell Renal Cell Carcinoma Through miR-484/ZEB1 Axis.长链非编码RNA PCED1B-AS1通过miR-484/ZEB1轴促进肾透明细胞癌进展。
Onco Targets Ther. 2021 Jan 13;14:393-402. doi: 10.2147/OTT.S270149. eCollection 2021.
7
LncRNA PCED1B-AS1 knockdown inhibits osteosarcoma via methylation-mediated miR-10a downregulation.LncRNA PCED1B-AS1 敲低通过甲基化介导的 miR-10a 下调抑制骨肉瘤。
J Orthop Surg Res. 2022 Oct 23;17(1):464. doi: 10.1186/s13018-022-03284-1.
8
LncRNA PCED1B-AS1 Upregulation in Hepatocellular Carcinoma and Regulation of the miR-10a/BCL6 Axis to Promote Cell Proliferation.长链非编码 RNA PCED1B-AS1 在肝癌中的上调及其对 miR-10a/BCL6 轴的调控促进细胞增殖。
Crit Rev Eukaryot Gene Expr. 2022;32(6):11-20. doi: 10.1615/CritRevEukaryotGeneExpr.2022039954.
9
LncRNA ST8SIA6-AS1 promotes colorectal cancer cell proliferation, migration and invasion by regulating the miR-5195/PCBP2 axis.长链非编码 RNA ST8SIA6-AS1 通过调控 miR-5195/PCBP2 轴促进结直肠癌细胞的增殖、迁移和侵袭。
Eur Rev Med Pharmacol Sci. 2020 Apr;24(8):4203-4211. doi: 10.26355/eurrev_202004_21000.
10
Long non-coding PCED1B-AS1 regulates macrophage apoptosis and autophagy by sponging miR-155 in active tuberculosis.长链非编码 PCED1B-AS1 通过海绵吸附 miR-155 调控结核分枝杆菌感染中巨噬细胞凋亡和自噬
Biochem Biophys Res Commun. 2019 Feb 12;509(3):803-809. doi: 10.1016/j.bbrc.2019.01.005. Epub 2019 Jan 6.

引用本文的文献

1
Integrative multi-omics analysis and experimental validation identify molecular subtypes, prognostic signature, and CA9 as a therapeutic target in oral squamous cell carcinoma.整合多组学分析与实验验证确定了口腔鳞状细胞癌的分子亚型、预后标志物以及作为治疗靶点的CA9。
Front Cell Dev Biol. 2025 Jul 9;13:1629683. doi: 10.3389/fcell.2025.1629683. eCollection 2025.
2
LncRNA PCED1B-AS1 mediates miR-3681-3p/MAP2K7 axis to promote metastasis, invasion and EMT in gastric cancer.长链非编码RNA PCED1B-AS1通过介导miR-3681-3p/MAP2K7轴促进胃癌的转移、侵袭和上皮-间质转化。
Biol Direct. 2024 May 2;19(1):34. doi: 10.1186/s13062-024-00468-z.
3

本文引用的文献

1
Updates on Clinical Use of Liquid Biopsy in Colorectal Cancer Screening, Diagnosis, Follow-Up, and Treatment Guidance.液体活检在结直肠癌筛查、诊断、随访及治疗指导中的临床应用进展
Front Cell Dev Biol. 2021 May 24;9:660924. doi: 10.3389/fcell.2021.660924. eCollection 2021.
2
An Integrated Analysis of the Response of Colorectal Adenocarcinoma Caco-2 Cells to X-Ray Exposure.结直肠癌Caco-2细胞对X射线照射反应的综合分析
Front Oncol. 2021 Jun 3;11:688919. doi: 10.3389/fonc.2021.688919. eCollection 2021.
3
Personalized medicine: Stem cells in colorectal cancer treatment.
Programmed cell death-ligand 2: new insights in cancer.
程序性细胞死亡配体 2：癌症的新见解。
Front Immunol. 2024 Mar 28;15:1359532. doi: 10.3389/fimmu.2024.1359532. eCollection 2024.
4
HOXA9 transcription factor is a double-edged sword: from development to cancer progression.HOXA9 转录因子是一把双刃剑：从发育到癌症进展。
Cancer Metastasis Rev. 2024 Jun;43(2):709-728. doi: 10.1007/s10555-023-10159-2. Epub 2023 Dec 8.
5
Upregulation of PCED1B-AS1 in proliferative diabetic retinopathy and its involvement in retinal vascular endothelial cell proliferation.PCED1B-AS1 在增殖性糖尿病视网膜病变中的上调及其在视网膜血管内皮细胞增殖中的作用。
BMC Ophthalmol. 2022 Nov 23;22(1):450. doi: 10.1186/s12886-022-02683-6.
6
Role of HOXA9 in solid tumors: mechanistic insights and therapeutic potential.HOXA9在实体瘤中的作用：机制洞察与治疗潜力
Cancer Cell Int. 2022 Nov 14;22(1):349. doi: 10.1186/s12935-022-02767-9.
7
Immune-related 3-lncRNA signature with prognostic connotation in a multi-cancer setting.免疫相关的 3-长链非编码 RNA 标志物在多癌种中的预后意义。
J Transl Med. 2022 Sep 30;20(1):442. doi: 10.1186/s12967-022-03654-7.
8
Identification of an m6A-Related Long Noncoding RNA Risk Model for Predicting Prognosis and Directing Treatments in Patients With Colon Adenocarcinoma.用于预测结肠腺癌患者预后及指导治疗的m6A相关长链非编码RNA风险模型的鉴定
Front Cell Dev Biol. 2022 Jul 13;10:910749. doi: 10.3389/fcell.2022.910749. eCollection 2022.
个性化医学：结直肠癌治疗中的干细胞。
Biomed Pharmacother. 2021 Sep;141:111821. doi: 10.1016/j.biopha.2021.111821. Epub 2021 Jun 16.
4
PRIMARY TUMOR LYMPHOVASCULAR INVASION NEGATIVELY AFFECTS SURVIVAL AFTER COLORECTAL LIVER METASTASIS RESECTION?结直肠肝转移切除术后原发性肿瘤脉管侵犯是否会影响生存？
Arq Bras Cir Dig. 2021 Jun 11;34(1):e1578. doi: 10.1590/0102-672020210001e1578. eCollection 2021.
5
Long non‑coding RNA PCED1B‑AS1 promotes pancreatic ductal adenocarcinoma progression by regulating the miR‑411‑3p/HIF‑1α axis.长链非编码 RNA PCED1B-AS1 通过调控 miR-411-3p/HIF-1α 轴促进胰腺导管腺癌进展。
Oncol Rep. 2021 Jul;46(1). doi: 10.3892/or.2021.8085. Epub 2021 May 26.
6
Long Non-Coding RNA PCED1B-AS1 Promotes the Progression of Clear Cell Renal Cell Carcinoma Through miR-484/ZEB1 Axis.长链非编码RNA PCED1B-AS1通过miR-484/ZEB1轴促进肾透明细胞癌进展。
Onco Targets Ther. 2021 Jan 13;14:393-402. doi: 10.2147/OTT.S270149. eCollection 2021.
7
MicroRNA-633 enhances melanoma cell proliferation and migration by suppressing KAI1.微小RNA-633通过抑制KAI1增强黑色素瘤细胞的增殖和迁移。
Oncol Lett. 2021 Feb;21(2):88. doi: 10.3892/ol.2020.12349. Epub 2020 Dec 6.
8
Long non‑coding RNA ZFPM2‑AS1 promotes colorectal cancer progression by sponging miR‑137 to regulate TRIM24.长链非编码 RNA ZFPM2-AS1 通过海绵吸附 miR-137 来调节 TRIM24 促进结直肠癌的进展。
Mol Med Rep. 2021 Feb;23(2). doi: 10.3892/mmr.2020.11737. Epub 2020 Dec 10.
9
Screening of T Cell-Related Long Noncoding RNA-MicroRNA-mRNA Regulatory Networks in Non-Small-Cell Lung Cancer.非小细胞肺癌中 T 细胞相关长非编码 RNA-微小 RNA-mRNA 调控网络的筛选。
Biomed Res Int. 2020 Nov 14;2020:5816763. doi: 10.1155/2020/5816763. eCollection 2020.
10
Dual targeting of PD-L1 and PD-L2 by PCED1B-AS1 via sponging hsa-miR-194-5p induces immunosuppression in hepatocellular carcinoma.PCED1B-AS1 通过海绵吸附 hsa-miR-194-5p 双重靶向 PD-L1 和 PD-L2，诱导肝癌免疫抑制。
Hepatol Int. 2021 Apr;15(2):444-458. doi: 10.1007/s12072-020-10101-6. Epub 2020 Nov 21.