Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences and Ōmura Satoshi Memorial Institute, Kitasato University, Tokyo, Japan.
Division of Clinical Laboratory, Sanritsu Zelkova Veterinary Laboratory, Tokyo, Japan.
J Vet Med Sci. 2023 Aug 1;85(8):858-866. doi: 10.1292/jvms.23-0165. Epub 2023 Jul 4.
The biotypic and genotypic features of Pasteurella canis isolated from dogs, cats, and humans were clarified by repetitive sequence-based fingerprinting and nucleotide sequences encoding trehalose-6-phosphate hydrolase (treC). Thirty P. canis and 48 P. multocida isolates were collected from dogs, cats, and humans to perform biotyping. The genotyping of P. canis by fingerprinting was followed by dendrogram construction. The whole-genome sequences (WGSs) were searched for the enzyme-coding nucleotide sequences around the main and adjacent loci constituting the operon. Full-length nucleotide sequences encoding the enzyme were determined using polymerase chain reaction and direct sequencing. Biotypic results were compared to the dendrogram and nucleotide sequence data. We observed a difference in trehalose fermentation with a positivity rate of 46.7%. Two (A-1/A-2) and three (B-1/B-2/B-3) clades were located on the dendrograms generated based on two repetitive sequence-based fingerprinting techniques, showing no association between trehalose fermentation and the clades. Based on the WGSs, two variants of the gene, namely, a 1,641 bp gene treC and a pseudogene (1,335 bp) of treC with its first 306 nucleotides deleted, were observed. Trehalose-positive isolates harbored treC, whereas trehalose-negative isolates lacked treC with or without the pseudogene. Our observations suggest biotypic and genotypic diversity among the P. canis isolates from animal and human hosts, with respect to trehalose fermentation and treC nucleotide sequences. This is the first report on the diversity of treC nucleotide sequences among these isolates.
从犬、猫和人分离的犬巴斯德菌的生物型和基因型特征通过重复序列指纹图谱和编码海藻糖-6-磷酸水解酶（treC）的核苷酸序列来阐明。从犬、猫和人收集了 30 株犬巴斯德菌和 48 株多杀巴斯德菌分离株进行生物分型。通过指纹图谱对犬巴斯德菌进行基因分型，然后构建聚类树。围绕构成操纵子的主要和相邻基因座搜索酶编码核苷酸序列的全基因组序列（WGS）。使用聚合酶链反应和直接测序确定酶的全长核苷酸序列。将生物型结果与聚类树和核苷酸序列数据进行比较。我们观察到海藻糖发酵的阳性率为 46.7%。在基于两种重复序列指纹图谱技术生成的聚类树上观察到两个（A-1/A-2）和三个（B-1/B-2/B-3）分支，海藻糖发酵与分支之间没有关联。基于 WGS，观察到基因的两个变体，即 1641 bp 的基因 treC 和缺失其前 306 个核苷酸的假基因（1335 bp）treC。海藻糖阳性分离株携带 treC，而海藻糖阴性分离株缺乏 treC，无论是否存在假基因。我们的观察结果表明，来自动物和人类宿主的犬巴斯德菌分离株在海藻糖发酵和 treC 核苷酸序列方面具有生物型和基因型多样性。这是首次报道这些分离株中 treC 核苷酸序列的多样性。
