Wang Yanxia, Chen Yao, Jiang Ling, Huang He
College of Food Science and Light Industry, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, China.
College of Food Science and Light Industry, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, China.
Biotechnol Adv. 2022 May-Jun;56:107927. doi: 10.1016/j.biotechadv.2022.107927. Epub 2022 Feb 16.
Mycotoxin contamination of food and feed is posing a serious threat to the global food safety and public health. Biological detoxification mediated by enzymes has emerged as a promising approach, as they can specifically degrade mycotoxins into non-toxic ones. However, the low degradation efficiency and stability limit their further application. To optimize the enzymes for mycotoxin removal, modification strategies that combine computational design with their structural data have been developed. Accordingly, this review will comprehensively summarize the recent trends in structure-based engineering to improve the enzyme catalytic efficiency, selectivity and stability in mycotoxins detoxification, which also provides perspectives in obtaining innovative and effective biocatalysts for mycotoxins degradation.
食品和饲料中的霉菌毒素污染对全球食品安全和公众健康构成了严重威胁。由酶介导的生物解毒作为一种有前景的方法已崭露头角，因为它们能够将霉菌毒素特异性地降解为无毒物质。然而，低降解效率和稳定性限制了它们的进一步应用。为了优化用于去除霉菌毒素的酶，已开发出将计算设计与其结构数据相结合的修饰策略。因此，本综述将全面总结基于结构的工程学的最新趋势，以提高酶在霉菌毒素解毒中的催化效率、选择性和稳定性，这也为获得用于霉菌毒素降解的创新且有效的生物催化剂提供了展望。
