Singh A, McFeters G A
Appl Environ Microbiol. 1986 Apr;51(4):738-42. doi: 10.1128/aem.51.4.738-742.1986.
Exposure of enterotoxigenic Escherichia coli strains to a sublethal concentration (0.75 mg/liter) of copper for 3 days at 4 degrees C induced sensitivity to deoxycholate (0.1%). When placed in a complex (brain heart infusion) or a defined amino acid salt medium, the copper-injured cells recovered their tolerance to deoxycholate in 3 and 6 h, respectively, and commenced active growth. Growth and heat-stable enterotoxin production of uninjured and copper-injured cells were studied in brain heart infusion medium. A slightly altered growth curve and an initial slow rate of toxin production were observed in injured cells when compared with those corresponding uninjured controls. However, maximum heat-stable enterotoxin levels in injured cultures were comparable to those produced by uninjured cells, suggesting that the enterotoxigenic potential of copper-injured cells was fully retained.
在4℃下，将产肠毒素大肠杆菌菌株暴露于亚致死浓度（0.75毫克/升）的铜中3天，可诱导其对脱氧胆酸盐（0.1%）敏感。当置于复合培养基（脑心浸液）或限定氨基酸盐培养基中时，受铜损伤的细胞分别在3小时和6小时恢复对脱氧胆酸盐的耐受性，并开始活跃生长。在脑心浸液培养基中研究了未损伤和受铜损伤细胞的生长及热稳定肠毒素产生情况。与相应的未损伤对照相比，损伤细胞的生长曲线略有改变，毒素产生的初始速率较慢。然而，损伤培养物中的最大热稳定肠毒素水平与未损伤细胞产生的水平相当，这表明受铜损伤细胞的产肠毒素潜力完全得以保留。
