Singh A, Yeager R, McFeters G A
Appl Environ Microbiol. 1986 Oct;52(4):832-7. doi: 10.1128/aem.52.4.832-837.1986.
Cells of one enteroinvasive and three enterotoxigenic strains of Escherichia coli were exposed to sublethal concentrations of copper and chlorine to produce 85 to 94% injury. Injured cells were intraluminally inoculated into ligated ileal loops of anesthetized mice, and injury was assessed at timed intervals. Substantial recovery (72-84%) of copper- and chlorine-injured cells was observed in the inoculated loops at 4 and 3 h, respectively. No appreciable increase in total numbers was observed during these time intervals. In vitro revival of copper-injured cells in phosphate-buffered saline alone after incubation at 35 degrees C for 4 h was not observed. However, a 60 to 70% revival occurred when 200 micrograms of protein per ml of mouse intestinal mucosal homogenate was incorporated into saline cell suspensions. The enterotoxigenic activity of copper-injured cells in rabbit ileal loops was somewhat reduced compared with that of chlorine-injured or uninjured cells. These results show that injured pathogenic E. coli cells can revive in the small intestine and appear to retain their enterotoxigenic activity.
将一株肠侵袭性大肠杆菌和三株产肠毒素大肠杆菌的细胞暴露于亚致死浓度的铜和氯中，造成85%至94%的损伤。将受损细胞经肠腔接种到麻醉小鼠的结扎回肠肠袢中，并在不同时间间隔评估损伤情况。在接种的肠袢中，分别在4小时和3小时观察到铜损伤和氯损伤细胞有显著恢复（72% - 84%）。在这些时间间隔内未观察到总数有明显增加。在35℃孵育4小时后，仅在磷酸盐缓冲盐水中铜损伤细胞未出现体外复苏。然而，当每毫升小鼠肠黏膜匀浆中加入200微克蛋白质到盐溶液细胞悬液中时，复苏率达到60%至70%。与氯损伤或未损伤细胞相比，铜损伤细胞在兔回肠肠袢中的产肠毒素活性有所降低。这些结果表明，受损的致病性大肠杆菌细胞可在小肠中复苏，并且似乎保留了它们的产肠毒素活性。
