Islam M Rafiqul, Rahman Md Mezanur, Mohi-Ud-Din Mohammed, Akter Munny, Zaman Erin, Keya Sanjida Sultana, Hasan Mehfuz, Hasanuzzaman Mirza
Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh.
Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, Texas, United States.
PeerJ. 2022 Feb 4;10:e12862. doi: 10.7717/peerj.12862. eCollection 2022.
Mungbean ( L. Wilczek) is one of the most important pulse crops, well-known for its protein-rich seeds. Growth and productivity are severely undermined by waterlogging.
In this study, we aim to evaluate how two promising phytohormones, namely cytokinin (CK) and gibberellic acid (GA), can improve waterlogging tolerance in mungbean by investigating key morphological, physiological, biochemical, and yield-related attributes.
Our results showed that foliar application of CK and GA under 5-day of waterlogged conditions improved mungbean growth and biomass, which was associated with increased levels of photosynthetic rate and pigments. Waterlogged-induced accumulation of reactive oxygen species and the consequently elevated levels of malondialdehyde were considerably reduced by CK and GA treatments. Mungbean plants sprayed with either CK or GA suffered less oxidative stress due to the enhancement of total phenolics and flavonoids levels. Improvement in the contents of proline and total soluble sugars indicated a better osmotic adjustment following CK and GA treatments in waterlogged-exposed plants. Most fundamentally, CK or GA-sprayed waterlogged-stressed mungbean plants demonstrated better performance in the aforementioned parameters after the 15-day recovery period as compared to water-sprayed waterlogged-exposed plants. Our results also revealed that CK and GA treatments increased yield-associated features in the waterlogged-stressed plant. Here, both phytohormones are efficient in improving mungbean resistance to waterlogging. However, CK was found to be more effective. Overall, our findings suggested that CK or GA could be used for managing waterlogging-induced damage to mungbean and perhaps in other cash crops.
绿豆（Vigna radiata (L.) Wilczek）是最重要的豆类作物之一，以其种子富含蛋白质而闻名。涝渍严重影响其生长和生产力。
在本研究中，我们旨在通过研究关键的形态、生理、生化和产量相关属性，评估两种有前景的植物激素，即细胞分裂素（CK）和赤霉素（GA），如何提高绿豆的耐涝性。
我们的结果表明，在5天的涝渍条件下叶面喷施CK和GA可改善绿豆生长和生物量，这与光合速率和色素水平的提高有关。CK和GA处理显著降低了涝渍诱导的活性氧积累以及随之升高的丙二醛水平。喷施CK或GA的绿豆植株由于总酚和类黄酮水平的提高而遭受的氧化应激较小。脯氨酸和总可溶性糖含量的改善表明，在遭受涝渍的植株中，CK和GA处理后渗透调节能力更好。最根本的是，与喷水的涝渍处理植株相比，喷施CK或GA的涝渍胁迫绿豆植株在15天恢复期后的上述参数表现更好。我们的结果还表明，CK和GA处理增加了涝渍胁迫植株的产量相关特征。在这里，两种植物激素都能有效提高绿豆的耐涝性。然而，发现CK更有效。总体而言，我们的研究结果表明，CK或GA可用于减轻涝渍对绿豆以及其他经济作物造成的损害。
