Pantoja Angles Aaron, Valle-Pérez Alexander U, Hauser Charlotte, Mahfouz Magdy M
Laboratory for Genome Engineering and Synthetic Biology, Division of Biological Sciences, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
Laboratory for Nanomedicine, Division of Biological Sciences, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
Front Bioeng Biotechnol. 2022 Feb 2;10:830200. doi: 10.3389/fbioe.2022.830200. eCollection 2022.
Many applications of synthetic biology require biological systems in engineered microbes to be delivered into diverse environments, such as for bioremediation, biosensing, and applications in medicine and agriculture. To avoid harming the target system (whether that is a farm field or the human gut), such applications require microbial biocontainment systems (MBSs) that inhibit the proliferation of engineered microbes. In the past decade, diverse molecular strategies have been implemented to develop MBSs that tightly control the proliferation of engineered microbes; this has enabled medical, industrial, and agricultural applications in which biological processes can be executed . The customization of MBSs also facilitate the integration of sensing modules for which different compounds can be produced and delivered upon changes in environmental conditions. These achievements have accelerated the generation of novel microbial systems capable of responding to external stimuli with limited interference from the environment. In this review, we provide an overview of the current approaches used for MBSs, with a specific focus on applications that have an immediate impact on multiple fields.
合成生物学的许多应用要求工程微生物中的生物系统能够被输送到各种不同的环境中，例如用于生物修复、生物传感以及医学和农业应用。为避免损害目标系统（无论是农田还是人体肠道），此类应用需要能够抑制工程微生物增殖的微生物生物遏制系统（MBS）。在过去十年中，人们实施了多种分子策略来开发能严格控制工程微生物增殖的MBS；这使得在医学、工业和农业领域能够执行生物过程的应用成为可能。MBS的定制还促进了传感模块的整合，在环境条件发生变化时能够产生并输送不同的化合物。这些成果加速了新型微生物系统的产生，这些系统能够在环境干扰有限的情况下对外界刺激做出反应。在本综述中，我们概述了目前用于MBS的方法，特别关注对多个领域有直接影响的应用。
