Department of Physics and Astronomy, University of Toledo, Toledo, OH 43606, USA.
Sensors (Basel). 2023 May 25;23(11):5053. doi: 10.3390/s23115053.
Fluorescence-based detection techniques are part of an ever-expanding field and are widely used in biomedical and environmental research as a biosensing tool. These techniques have high sensitivity, selectivity, and a short response time, making them a valuable tool for developing bio-chemical assays. The endpoint of these assays is defined by changes in fluorescence signal, in terms of its intensity, lifetime, and/or shift in spectrum, which is monitored using readout devices such as microscopes, fluorometers, and cytometers. However, these devices are often bulky, expensive, and require supervision to operate, which makes them inaccessible in resource-limited settings. To address these issues, significant effort has been directed towards integrating fluorescence-based assays into miniature platforms based on papers, hydrogels, and microfluidic devices, and to couple these assays with portable readout devices like smartphones and wearable optical sensors, thereby enabling point-of-care detection of bio-chemical analytes. This review highlights some of the recently developed portable fluorescence-based assays by discussing the design of fluorescent sensor molecules, their sensing strategy, and the fabrication of point-of-care devices.
基于荧光的检测技术是一个不断发展的领域的一部分，被广泛应用于生物医学和环境研究中，作为生物传感工具。这些技术具有高灵敏度、选择性和短的响应时间，使其成为开发生物化学分析的有价值的工具。这些分析的终点是通过荧光信号的变化来定义的，包括强度、寿命和/或光谱的位移，这些变化是使用显微镜、荧光计和细胞计等读出设备来监测的。然而，这些设备通常体积庞大、昂贵，并且需要监督操作，这使得它们在资源有限的环境中无法使用。为了解决这些问题，人们已经做出了很大的努力，将基于纸张、水凝胶和微流控设备的荧光分析集成到微型平台中，并将这些分析与智能手机和可穿戴光学传感器等便携式读出设备相结合，从而实现生物化学分析物的即时检测。本综述通过讨论荧光传感器分子的设计、它们的传感策略以及即时检测设备的制造，强调了一些最近开发的便携式基于荧光的分析。
