Center for Advanced Biomaterials for Healthcare@CRIB, Istituto Italiano di Tecnologia (IIT), Largo Barsanti e Matteucci 53, 80125 Naples, Italy.
Dipartimento di Ingegneria Chimica del Materiali e della Produzione Industriale (DICMAPI), University Federico II, Piazzale Tecchio 80, 80125 Naples, Italy.
J R Soc Interface. 2022 Feb;19(187):20210800. doi: 10.1098/rsif.2021.0800. Epub 2022 Feb 23.
In cancer therapy, stimulus-responsive drug delivery systems are of particular interest for reducing side effects in healthy tissues and improving drug selectivity in the tumoral ones. Here, a strategy for the preparation of a photo-responsive cross-linked trilayer deposited onto an oil-in-water nanoemulsion via a layer-by-layer technique is reported. The system is made of completely biocompatible materials such as soybean oil, egg lecithin and glycol chitosan, with heparin as the polymeric shell. The oil core is pre-loaded with curcumin as a model lipophilic active molecule with anti-tumoral properties. The trilayer cross-linkage is performed via a photoinitiator-free thiol-ene 'click' reaction. In particular, the system is implemented with an -nitrobenzyl group functionalized with a thiol moiety which can perform both the thiol-ene 'click' reaction and the cleavage meant for controlled drug release at two different wavelengths, respectively. So the preparation and characterization of a photo-responsive natural nanocarrier (PNC) that is stable under physiological conditions owing to the thiol-ene cross-linkage are reported. PNC performance has been assessed on melanoma cells as well as on xenograft tumour-induced mice.
在癌症治疗中，刺激响应药物传递系统对于减少健康组织的副作用和提高肿瘤组织的药物选择性特别感兴趣。在这里，报道了一种通过层层技术将光响应交联的三层沉积在油包水纳米乳液上的制备策略。该系统由完全生物相容的材料组成，如大豆油、蛋黄卵磷脂和乙二醇壳聚糖，肝素作为聚合物壳。油芯预先加载姜黄素作为具有抗肿瘤特性的模型亲脂性活性分子。三层交联通过无光引发剂的硫醇-烯“点击”反应进行。特别是，该系统采用了带有巯基部分的 -硝基苄基官能化，该部分可以分别进行硫醇-烯“点击”反应和用于控制药物释放的裂解，以实现两种不同的波长。因此，报道了一种光响应天然纳米载体（PNC）的制备和表征，由于硫醇-烯交联，该载体在生理条件下稳定。已经评估了 PNC 在黑色素瘤细胞以及异种移植肿瘤诱导的小鼠上的性能。
