• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Cy5标记的巯基化和甲基化壳聚糖-羧甲基葡聚糖纳米颗粒在视网膜母细胞瘤动物模型中的生物分布

Biodistribution of Cy5-labeled Thiolated and Methylated Chitosan-Carboxymethyl Dextran Nanoparticles in an Animal Model of Retinoblastoma.

作者信息

Delrish Elham, Ghassemi Fariba, Jabbarvand Mahmoud, Lashay Alireza, Atyabi Fatemeh, Soleimani Masoud, Dinarvand Rassoul

机构信息

Translational Ophthalmology Research Centre, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Retina & Vitreous Service, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

J Ophthalmic Vis Res. 2022 Jan 21;17(1):58-68. doi: 10.18502/jovr.v17i1.10171. eCollection 2022 Jan-Mar.

DOI:10.18502/jovr.v17i1.10171
PMID:35194497
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8850845/
Abstract

PURPOSE

The use of more potent medicine for local chemotherapy of retinoblastoma in order to minimize local and systemic adverse effects is essential. The main goal of this investigation was to assess the biodistribution of thiolated and methylated chitosan-carboxymethyl dextran nanoparticles (CMD-TCs-NPs and CMD-TMC-NPs) following intravitreal (IVT) injection into rat eyes with retinoblastoma.

METHODS

An ionic gelation method was used to fabricate Cy5-labelled CMD-TCs-NPs and CMD-TMC-NPs. The NPs were characterized. Cellular internalization of Cy5-labelled NPs was investigated using confocal microscopy and the absorption of labeled NPs was quantified by flow cytometry in human retinoblastoma (Y79) cells. In addition, the Cy5-labeled distribution of nanoparticles in the posterior segment of the eye was histologically imaged by confocal microscopy after IVT injection of NPs into the eyes of rats with retinoblastoma.

RESULTS

CMD-TCs-NPs and CMD-TMC-NPs showed a mean diameter of 34 3.78 nm and 42 4.23 nm and zeta potential of +11 2.27 mV and +29 4.31mV, respectively. The in vivo study of intraocular biodistribution of Cy5-labeled CMD-TCs-NPs and CMD-TMC-NPs revealed that there is more affinity of CMD-TCs-NPs to the retina and retinoblastoma tumor after IVT administration while methylated chitosan nanoparticles are immobilized in the vitreous and are not able to reach the retina even after 24 hr.

CONCLUSION

The ionic gelation technique was efficient in synthesizing a biocompatible polymeric nanosystem for drug delivery into the posterior segment of the eye. The current study demonstrated increased ocular bioavailability of CMD-TCs-NPs relative to CMD-TMC-NPs in retinoblastoma induced rat eyes.

摘要

目的

使用更强效的药物进行视网膜母细胞瘤的局部化疗以尽量减少局部和全身不良反应至关重要。本研究的主要目的是评估硫醇化和甲基化壳聚糖 - 羧甲基葡聚糖纳米颗粒（CMD - TCs - NPs和CMD - TMC - NPs）玻璃体内（IVT）注射入患有视网膜母细胞瘤的大鼠眼后其生物分布情况。

方法

采用离子凝胶法制备Cy5标记的CMD - TCs - NPs和CMD - TMC - NPs。对纳米颗粒进行表征。使用共聚焦显微镜研究Cy5标记纳米颗粒的细胞内化情况，并通过流式细胞术对人视网膜母细胞瘤（Y79）细胞中标记纳米颗粒的摄取进行定量。此外，将纳米颗粒玻璃体内注射入患有视网膜母细胞瘤的大鼠眼后，通过共聚焦显微镜对纳米颗粒在眼后段的Cy5标记分布进行组织学成像。

结果

CMD - TCs - NPs和CMD - TMC - NPs的平均直径分别为34 ± 3.78 nm和42 ± 4.23 nm，zeta电位分别为 +11 ± 2.27 mV和 +29 ± 4.31 mV。Cy5标记的CMD - TCs - NPs和CMD - TMC - NPs眼内生物分布的体内研究表明，玻璃体内给药后，CMD - TCs - NPs对视网膜和视网膜母细胞瘤肿瘤的亲和力更高，而甲基化壳聚糖纳米颗粒固定在玻璃体中，即使在24小时后也无法到达视网膜。

结论

离子凝胶技术有效地合成了一种用于将药物递送至眼后段的生物相容性聚合物纳米系统。当前研究表明，在视网膜母细胞瘤诱导的大鼠眼中，CMD - TCs - NPs相对于CMD - TMC - NPs具有更高的眼内生物利用度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c5/8850845/9b839ab6cb7a/jovr-17-58-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c5/8850845/7e4fa0074b37/jovr-17-58-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c5/8850845/43605a294f81/jovr-17-58-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c5/8850845/f5babaf7720d/jovr-17-58-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c5/8850845/58f2499ebe51/jovr-17-58-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c5/8850845/1168291e4af5/jovr-17-58-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c5/8850845/9b839ab6cb7a/jovr-17-58-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c5/8850845/7e4fa0074b37/jovr-17-58-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c5/8850845/43605a294f81/jovr-17-58-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c5/8850845/f5babaf7720d/jovr-17-58-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c5/8850845/58f2499ebe51/jovr-17-58-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c5/8850845/1168291e4af5/jovr-17-58-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c5/8850845/9b839ab6cb7a/jovr-17-58-g006.jpg

相似文献

1
Biodistribution of Cy5-labeled Thiolated and Methylated Chitosan-Carboxymethyl Dextran Nanoparticles in an Animal Model of Retinoblastoma.Cy5标记的巯基化和甲基化壳聚糖-羧甲基葡聚糖纳米颗粒在视网膜母细胞瘤动物模型中的生物分布
J Ophthalmic Vis Res. 2022 Jan 21;17(1):58-68. doi: 10.18502/jovr.v17i1.10171. eCollection 2022 Jan-Mar.
2
The Antitumor Effect of Topotecan Loaded Thiolated Chitosan-Dextran Nanoparticles for Intravitreal Chemotherapy: A Xenograft Retinoblastoma Model.用于玻璃体内化疗的载有拓扑替康的硫醇化壳聚糖-葡聚糖纳米颗粒的抗肿瘤作用：异种移植视网膜母细胞瘤模型
J Ophthalmic Vis Res. 2023 Feb 21;18(1):68-80. doi: 10.18502/jovr.v18i1.12727. eCollection 2023 Jan-Mar.
3
Efficacy of topotecan nanoparticles for intravitreal chemotherapy of retinoblastoma.拓扑替康纳米粒用于视网膜母细胞瘤眼内化疗的疗效。
Exp Eye Res. 2021 Mar;204:108423. doi: 10.1016/j.exer.2020.108423. Epub 2021 Jan 13.
4
Erratum: Biodistribution of Cy5-labeled Thiolated and Methylated Chitosan-Carboxymethyl Dextran Nanoparticles in an Animal Model of Retinoblastoma.勘误：Cy5标记的巯基化和甲基化壳聚糖-羧甲基葡聚糖纳米颗粒在视网膜母细胞瘤动物模型中的生物分布。
J Ophthalmic Vis Res. 2022 Apr 29;17(2):158-159. doi: 10.18502/jovr.v17i2.10826. eCollection 2022 Apr-Jun.
5
Preparation of stable chitosan-carboxymethyl dextran nanoparticles.稳定的壳聚糖-羧甲基葡聚糖纳米颗粒的制备
J Nanosci Nanotechnol. 2009 Apr;9(4):2558-65. doi: 10.1166/jnn.2009.222.
6
Intranasal delivery of Huperzine A to the brain using lactoferrin-conjugated N-trimethylated chitosan surface-modified PLGA nanoparticles for treatment of Alzheimer's disease.使用乳铁蛋白共轭的N-三甲基化壳聚糖表面修饰的聚乳酸-羟基乙酸共聚物纳米颗粒经鼻向脑递送石杉碱甲用于治疗阿尔茨海默病。
Int J Nanomedicine. 2018 Feb 1;13:705-718. doi: 10.2147/IJN.S151474. eCollection 2018.
7
Synthesis of thiolated chitosan and preparation nanoparticles with sodium alginate for ocular drug delivery.硫醇化壳聚糖的合成及与海藻酸钠制备用于眼部给药的纳米颗粒。
Mol Vis. 2012;18:1973-82. Epub 2012 Jul 18.
8
Intraocular biodistribution of intravitreal injected chitosan/gelatin nanoparticles.玻璃体内注射壳聚糖/明胶纳米颗粒的眼内生物分布。
Rom J Morphol Embryol. 2014;55(3):869-75.
9
Combinatorial anticancer effects of curcumin and 5-fluorouracil loaded thiolated chitosan nanoparticles towards colon cancer treatment.姜黄素和5-氟尿嘧啶负载的巯基化壳聚糖纳米粒对结肠癌治疗的联合抗癌作用。
Biochim Biophys Acta. 2014 Sep;1840(9):2730-43. doi: 10.1016/j.bbagen.2014.06.004. Epub 2014 Jun 17.
10
INTRAOCULAR BIODISTRIBUTION OF INTRAVITREAL INJECTED FLUORESCENT DEXAMETHASONE-CHITOSAN NANOPARTICLES IN RABBIT EYES.兔眼玻璃体内注射荧光地塞米松-壳聚糖纳米颗粒的眼内生物分布
Rev Med Chir Soc Med Nat Iasi. 2015 Apr-Jun;119(2):484-90.

引用本文的文献

1
The Contribution of Nanomedicine in Ocular Oncology.纳米医学在眼部肿瘤学中的贡献。
Cancers (Basel). 2025 Mar 31;17(7):1186. doi: 10.3390/cancers17071186.
2
Advancements in Nanosystems for Ocular Drug Delivery: A Focus on Pediatric Retinoblastoma.纳米系统在眼部药物输送中的进展：以小儿视网膜母细胞瘤为重点。
Molecules. 2024 May 11;29(10):2263. doi: 10.3390/molecules29102263.
3
An Update on Emergent Nano-Therapeutic Strategies against Pediatric Brain Tumors.小儿脑肿瘤紧急纳米治疗策略的最新进展

本文引用的文献

1
Strategies for Improving Ocular Drug Bioavailability and Corneal Wound Healing with Chitosan-Based Delivery Systems.基于壳聚糖的给药系统改善眼部药物生物利用度和角膜伤口愈合的策略
Polymers (Basel). 2018 Nov 3;10(11):1221. doi: 10.3390/polym10111221.
2
Insight into Cellular Uptake and Intracellular Trafficking of Nanoparticles.纳米颗粒的细胞摄取与细胞内运输洞察
Nanoscale Res Lett. 2018 Oct 25;13(1):339. doi: 10.1186/s11671-018-2728-6.
3
A Broad-Spectrum ROS-Eliminating Material for Prevention of Inflammation and Drug-Induced Organ Toxicity.
Brain Sci. 2024 Feb 18;14(2):185. doi: 10.3390/brainsci14020185.
4
Next-Generation Nanomedicine Approaches for the Management of Retinal Diseases.用于视网膜疾病治疗的新一代纳米医学方法。
Pharmaceutics. 2023 Jul 22;15(7):2005. doi: 10.3390/pharmaceutics15072005.
5
Biodegradable Biopolymeric Nanoparticles for Biomedical Applications-Challenges and Future Outlook.用于生物医学应用的可生物降解生物聚合物纳米颗粒——挑战与未来展望
Materials (Basel). 2023 Mar 15;16(6):2364. doi: 10.3390/ma16062364.
6
Sustainable Biodegradable Biopolymer-Based Nanoparticles for Healthcare Applications.用于医疗保健应用的可持续生物可降解生物基纳米粒子。
Int J Mol Sci. 2023 Feb 6;24(4):3188. doi: 10.3390/ijms24043188.
7
Nanotechnology for Pediatric Retinoblastoma Therapy.用于小儿视网膜母细胞瘤治疗的纳米技术
Pharmaceuticals (Basel). 2022 Aug 31;15(9):1087. doi: 10.3390/ph15091087.
8
Erratum: Biodistribution of Cy5-labeled Thiolated and Methylated Chitosan-Carboxymethyl Dextran Nanoparticles in an Animal Model of Retinoblastoma.勘误：Cy5标记的巯基化和甲基化壳聚糖-羧甲基葡聚糖纳米颗粒在视网膜母细胞瘤动物模型中的生物分布。
J Ophthalmic Vis Res. 2022 Apr 29;17(2):158-159. doi: 10.18502/jovr.v17i2.10826. eCollection 2022 Apr-Jun.
9
Thiolated Chitosan-carboxymethyl Dextran Nanoparticles: Improving Intravitreal Drug Bioavailability for Retinoblastoma.硫醇化壳聚糖-羧甲基葡聚糖纳米颗粒：提高视网膜母细胞瘤玻璃体腔内药物的生物利用度
J Ophthalmic Vis Res. 2022 Jan 21;17(1):1-3. doi: 10.18502/jovr.v17i1.10163. eCollection 2022 Jan-Mar.
一种用于预防炎症和药物诱导的器官毒性的广谱活性氧清除材料。
Adv Sci (Weinh). 2018 Aug 16;5(10):1800781. doi: 10.1002/advs.201800781. eCollection 2018 Oct.
4
Modified hyaluronic acid based materials for biomedical applications.用于生物医学应用的改性透明质酸基材料。
Int J Biol Macromol. 2019 Jan;121:556-571. doi: 10.1016/j.ijbiomac.2018.10.049. Epub 2018 Oct 12.
5
A carboxymethyl dextran-based polymeric conjugate as the antigen carrier for cancer immunotherapy.一种基于羧甲基葡聚糖的聚合物偶联物作为癌症免疫治疗的抗原载体。
Biomater Res. 2018 Aug 14;22:21. doi: 10.1186/s40824-018-0131-0. eCollection 2018.
6
Preparation and Characterization of Quaternized Chitosan Derivatives and Assessment of Their Antioxidant Activity.季铵化壳聚糖衍生物的制备及性质表征与其抗氧化活性评价。
Molecules. 2018 Feb 26;23(3):516. doi: 10.3390/molecules23030516.
7
Hyaluronic Acid-Based Micelles as Ocular Platform to Modulate the Loading, Release, and Corneal Permeation of Corticosteroids.基于透明质酸的胶束作为眼部平台调节皮质类固醇的载药、释放和角膜渗透。
Macromol Biosci. 2017 Dec;17(12). doi: 10.1002/mabi.201700261. Epub 2017 Nov 16.
8
N,N,N-Trimethyl chitosan: An advanced polymer with myriad of opportunities in nanomedicine.三甲基壳聚糖：一种具有众多纳米医学应用机会的先进聚合物。
Carbohydr Polym. 2017 Feb 10;157:875-902. doi: 10.1016/j.carbpol.2016.10.041. Epub 2016 Oct 19.
9
Effective Retinal Penetration of Lipophilic and Lipid-Conjugated Hydrophilic Agents Delivered by Engineered Liposomes.工程化脂质体递送的亲脂性和亲脂共轭亲水性药物的有效视网膜穿透性
Mol Pharm. 2017 Feb 6;14(2):423-430. doi: 10.1021/acs.molpharmaceut.6b00864. Epub 2016 Dec 27.
10
Hyaluronic acid-coated liposomes for targeted delivery of paclitaxel, in-vitro characterization and in-vivo evaluation.透明质酸包覆脂质体用于紫杉醇的靶向递送：体外特性分析和体内评价。
J Control Release. 2016 May 10;229:10-22. doi: 10.1016/j.jconrel.2016.03.012. Epub 2016 Mar 9.