在大肠杆菌和鼠伤寒沙门氏菌中，leuABCD上游DNA序列中高A+T含量保守。
High A + T content conserved in DNA sequences upstream of leuABCD in Escherichia coli and Salmonella typhimurium.
作者信息
Haughn G W, Wessler S R, Gemmill R M, Calvo J M
出版信息
J Bacteriol. 1986 Jun;166(3):1113-7. doi: 10.1128/jb.166.3.1113-1117.1986.
Abstract
The nucleotide sequence of over 800 base pairs of DNA upstream of leuP was determined for Escherichia coli and Salmonella typhimurium. In both of these enteric bacteria, approximately 500 base pairs of A + T-rich sequences separates leuP from an upstream open reading frame. Although these A + T-rich sequences share little homology, the distribution of A + T base pairs within the region is strikingly conserved. Deletion of the A + T-rich sequences upstream of the E. coli leu operon does not markedly affect the strength of the leu promoter in vivo.
摘要
测定了大肠杆菌和鼠伤寒沙门氏菌亮氨酸操纵子上游超过800个碱基对的DNA核苷酸序列。在这两种肠道细菌中，约500个富含A+T的碱基对序列将亮氨酸操纵子与上游的一个开放阅读框分隔开来。尽管这些富含A+T的序列同源性很低，但该区域内A+T碱基对的分布却显著保守。删除大肠杆菌亮氨酸操纵子上游富含A+T的序列，在体内对亮氨酸启动子的强度没有明显影响。
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