Geary C, Stevens M
J Clin Microbiol. 1986 Jun;23(6):1044-5. doi: 10.1128/jcm.23.6.1044-1045.1986.
A rapid, simple lysostaphin lysis susceptibility test to differentiate the genera Staphylococcus and Micrococcus was evaluated. Of 181 strains from culture collections, 95 of 95 Staphylococcus strains were lysed, and 79 of 79 Micrococcus strains were not lysed. The seven Planococcus strains were resistant. Clinical isolates (890) were tested with lysostaphin and for the ability to produce acid from glycerol in the presence of erythromycin. Overall agreement between the methods was 99.2%. All clinical Micrococcus strains (43) were resistant to lysostaphin, and all clinical Staphylococcus strains (847) were susceptible. Seven of the Staphylococcus strains did not produce acid from glycerol in the presence of erythromycin. This lysostaphin test provides results in 2 h. It is easier to perform than previously described lysostaphin lysis methods. It is also more rapid and accurate than the glycerol-erythromycin test.
对一种用于区分葡萄球菌属和微球菌属的快速、简单的溶葡萄球菌素裂解敏感性试验进行了评估。在来自菌种保藏库的181株菌株中，95株葡萄球菌菌株全部被裂解，79株微球菌菌株均未被裂解。7株动性球菌菌株具有抗性。对890株临床分离株进行了溶葡萄球菌素检测以及在红霉素存在的情况下从甘油产酸能力的检测。两种方法的总体一致性为99.2%。所有临床微球菌菌株（43株）对溶葡萄球菌素均有抗性，所有临床葡萄球菌菌株（847株）均敏感。7株葡萄球菌菌株在红霉素存在的情况下不能从甘油产酸。这种溶葡萄球菌素试验在2小时内即可得出结果。它比先前描述的溶葡萄球菌素裂解方法更易于操作。它也比甘油 - 红霉素试验更快且更准确。
