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用于鉴定金黄色葡萄球菌的乳胶试验的比较评估

Comparative evaluation of a latex test for the identification of Staphylococcus aureus.

作者信息

Stevens M, Geary C

机构信息

Public Health Laboratory, Royal Infirmary, Leicester, UK.

出版信息

Eur J Clin Microbiol Infect Dis. 1989 Feb;8(2):153-6. doi: 10.1007/BF01963902.

DOI:10.1007/BF01963902
PMID:2498100
Abstract

A rapid latex agglutination test, Staphaurex, was tested for its ability to identify Staphylococcus aureus using 72 reference strains and 785 clinical isolates of the family Micrococcaceae. All reference strains of Staphylococcus aureus were Staphaurex-positive. Non-Staphylococcus aureus reference strains were negative. Using clinical strains, the results of the Staphaurex test were compared with the results of other tests commonly used to identify Staphylococcus aureus. A total of 393 clinical isolates were classified as Staphylococcus aureus. The Staphaurex, slide coagulase, tube coagulase/human plasma and tube coagulase/rabbit plasma tests correctly identified 98%, 93.6%, 93.6% and 97.5% of the Staphylococcus aureus strains, respectively. The performance of the Staphaurex test, in terms of sensitivity and specificity, was significantly better than the slide coagulase test. It was as sensitive and almost as specific as the tube coagulase rabbit test and more sensitive than the tube coagulase human test.

摘要

采用一种快速乳胶凝集试验——葡萄球菌快速诊断试剂(Staphaurex),以72株参考菌株和785株微球菌科临床分离株为样本,检测其鉴定金黄色葡萄球菌的能力。所有金黄色葡萄球菌参考菌株的葡萄球菌快速诊断试剂检测结果均为阳性。非金黄色葡萄球菌参考菌株检测结果为阴性。使用临床菌株,将葡萄球菌快速诊断试剂检测结果与其他常用于鉴定金黄色葡萄球菌的检测结果进行比较。共有393株临床分离株被分类为金黄色葡萄球菌。葡萄球菌快速诊断试剂、玻片凝固酶、试管凝固酶/人血浆和试管凝固酶/兔血浆试验分别正确鉴定出98%、93.6%、93.6%和97.5%的金黄色葡萄球菌菌株。在敏感性和特异性方面,葡萄球菌快速诊断试剂检测的性能显著优于玻片凝固酶试验。其敏感性与试管凝固酶兔血浆试验相当,特异性几乎相同,且比试管凝固酶人血浆试验更敏感。

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本文引用的文献

1
Rapid and reliable identification of Staphylococcus aureus by a latex agglutination test.通过乳胶凝集试验快速可靠地鉴定金黄色葡萄球菌。
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Evaluation of three commercial agglutination tests for the identification of Staphylococcus aureus.用于鉴定金黄色葡萄球菌的三种商业凝集试验的评估
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Rate of isolation of Staphylococcus aureus strains possessing coagulase or clumping factor.具有凝固酶或聚集因子的金黄色葡萄球菌菌株的分离率。
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Evaluation of rapid coagulase methods for the identification of Staphylococcus aureus.用于鉴定金黄色葡萄球菌的快速凝固酶方法的评估。
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Coagulase production by strains of Staphylococcus aureus of differing resistance characters: a comparison of two traditional methods with a latex agglutination system detecting both clumping factor and protein A.不同耐药特性的金黄色葡萄球菌菌株的凝固酶产生:两种传统方法与一种可检测聚集因子和蛋白A的乳胶凝集系统的比较
J Clin Pathol. 1986 Apr;39(4):371-5. doi: 10.1136/jcp.39.4.371.
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Rapid lysostaphin test to differentiate Staphylococcus and Micrococcus species.用于区分葡萄球菌属和微球菌属菌种的快速溶葡萄球菌素试验。
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Single-tube mixed agglutination test for the detection of staphylococcal protein A.用于检测葡萄球菌蛋白A的单管混合凝集试验。
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