Gibbons G F, Howard D H
J Gen Microbiol. 1986 Feb;132(2):263-8. doi: 10.1099/00221287-132-2-263.
Auxotrophic mutants of Candida albicans FC18 were induced by a combination of treatments with nitrous acid and UV irradiation. Arginine (Arg-), histidine (His-) and methionine/cysteine (MetA-) auxotrophs were recovered by this means. The Arg- auxotrophs lacked active argininosuccinate lyase (EC 4.3.2.1), the enzyme catalysing the final step in arginine biosynthesis. Thus the locus may be designated arg-4. The mutant strains bearing this mutation did not form germ tubes unless the germination medium contained arginine.
白色念珠菌FC18的营养缺陷型突变体通过亚硝酸处理和紫外线照射相结合的方法诱导产生。通过这种方式获得了精氨酸（Arg-）、组氨酸（His-）和蛋氨酸/半胱氨酸（MetA-）营养缺陷型菌株。精氨酸营养缺陷型菌株缺乏活性精氨琥珀酸裂解酶（EC 4.3.2.1），该酶催化精氨酸生物合成的最后一步。因此，该基因座可命名为arg-4。携带这种突变的突变菌株除非在发芽培养基中含有精氨酸，否则不会形成芽管。
