Balish E
Appl Microbiol. 1973 Apr;25(4):615-20. doi: 10.1128/am.25.4.615-620.1973.
A prototrophic strain and 21 auxotrophic strains of Candida albicans were assessed for their capacity to produce chlamydospores and germ tubes. All of the mutants were able to produce germ-tubes in human serum but only two mutants produced them in defined medium with L-alpha-amino-n-butyric acid as the sole source of nitrogen. Most auxotrophs were not able to produce chlamydospores on corn meal agar with 1% Tween 80, but they could be induced to do so if the medium was supplemented with their growth requirement(s). Although L-cysteine was able to support the growth of two methionine mutants, it did not support chlamydospore formation when added to corn meal agar with 1% Tween 80. Mutants of C. albicans that do not form chlamydospores could be incorrectly identified in laboratories that rely on chlamydospore formation for identification.
对白色念珠菌的1株原养型菌株和21株营养缺陷型菌株产生厚垣孢子和芽管的能力进行了评估。所有突变体都能够在人血清中产生芽管，但只有两个突变体能够在以L-α-氨基-n-丁酸作为唯一氮源的限定培养基中产生芽管。大多数营养缺陷型菌株在添加1%吐温80的玉米粉琼脂上不能产生厚垣孢子，但如果在培养基中补充它们的生长需求，则可以诱导它们产生厚垣孢子。虽然L-半胱氨酸能够支持两个甲硫氨酸突变体的生长，但当添加到含有1%吐温80的玉米粉琼脂中时，它并不支持厚垣孢子的形成。在依靠厚垣孢子形成进行鉴定的实验室中，不形成厚垣孢子的白色念珠菌突变体可能会被错误鉴定。
