白色念珠菌的遗传学分析：多种营养缺陷型的分离以及连锁和互补的证明。
A genetic analysis of Candida albicans: isolation of a wide variety of auxotrophs and demonstration of linkage and complementation.
作者信息
Kakar S N, Partridge R M, Magee P T
出版信息
Genetics. 1983 Jun;104(2):241-55. doi: 10.1093/genetics/104.2.241.
Abstract
Naturally occurring strains of Candida albicans appear to be diploid and heterozygous for a limited number of nutritional markers. Additional heterozygosity can be induced by treatment with mutagens; nitrous acid alone or in combination with UV is a potent mutagen in terms of both efficacy and efficiency in the production of a wide variety of mutations. Spheroplast fusion followed by regeneration on selective media revealed complementation among four histidine-requiring mutants analyzed. Some of the fusion products appeared to be stable prototrophs, whereas in others several kinds of segregants resulted, apparently due to chromosomal or nuclear elimination. The results are suggestive of both heterokaryosis as well as nuclear fusion. The procedures described can be successfully used for generating new mutants and studying allelism. Three sets of linkage relationships have been derived from evidence provided by concomitant appearance or cosegragation of several auxotrophic markers.
摘要
白色念珠菌的自然菌株似乎是二倍体，并且在有限数量的营养标记上是杂合的。用诱变剂处理可诱导额外的杂合性；就产生多种突变的效力和效率而言，亚硝酸单独使用或与紫外线联合使用都是一种有效的诱变剂。原生质球融合后在选择培养基上再生，揭示了所分析的四个组氨酸需求突变体之间的互补作用。一些融合产物似乎是稳定的原养型，而在其他一些融合产物中则产生了几种类型的分离子，这显然是由于染色体或核的消除。这些结果提示了异核体形成以及核融合。所描述的程序可成功用于产生新的突变体并研究等位性。从几个营养缺陷型标记的同时出现或共分离所提供的证据中得出了三组连锁关系。
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