Department of Biosciences, Rice University MS-140, 6100 Main St., Houston, TX 77005, USA.
Department of Bioengineering, Rice University MS-140, 6100 Main St., Houston, TX 77005, USA.
Biosensors (Basel). 2022 Jan 25;12(2):64. doi: 10.3390/bios12020064.
In recent years, small-molecule biosensors have become increasingly important in synthetic biology and biochemistry, with numerous new applications continuing to be developed throughout the field. For many biosensors, however, their utility is hindered by poor functionality. Here, we review the known types of mechanisms of biosensors within bacterial cells, and the types of approaches for optimizing different biosensor functional parameters. Discussed approaches for improving biosensor functionality include methods of directly engineering biosensor genes, considerations for choosing genetic reporters, approaches for tuning gene expression, and strategies for incorporating additional genetic modules.
近年来，小分子生物传感器在合成生物学和生物化学领域变得越来越重要，该领域不断有新的应用被开发出来。然而，对于许多生物传感器来说，其功能的实用性受到阻碍。在这里，我们回顾了细菌细胞内生物传感器的已知机制类型，以及优化不同生物传感器功能参数的方法类型。讨论的用于提高生物传感器功能的方法包括直接工程生物传感器基因的方法、选择遗传报告基因的考虑因素、基因表达调整的方法以及整合额外遗传模块的策略。
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