以汽巴克隆蓝F3GA作为通用装配抑制剂对体外核糖体装配动力学序列的研究。

Datta D, Changchien L M, Craven G R

Nucleic Acids Res. 1986 May 27;14(10):4095-111. doi: 10.1093/nar/14.10.4095.

We have found that all E. coli ribosomal proteins strongly bind to an agarose affinity column derivatized with the dye Cibacron Blue F3GA. We have also shown that the capacity to bind the dye is lost when the proteins are organized within the structure of the ribosome or are members of pre-formed protein-RNA complexes. We conclude that the binding of ribosomal proteins to this dye involves specific protein-RNA recognition sites. These observations led us to discover that Cibacron Blue can be used to inhibit in vitro ribosome assembly at any stage of the assembly process. This has allowed us to determine a kinetic order of ribosome assembly.

我们发现，所有大肠杆菌核糖体蛋白都能与用染料Cibacron Blue F3GA衍生化的琼脂糖亲和柱紧密结合。我们还表明，当这些蛋白在核糖体结构中组织起来或成为预先形成的蛋白质-RNA复合物的成员时，与染料结合的能力就会丧失。我们得出结论，核糖体蛋白与这种染料的结合涉及特定的蛋白质-RNA识别位点。这些观察结果使我们发现，Cibacron Blue可用于在体外核糖体组装过程的任何阶段抑制核糖体组装。这使我们能够确定核糖体组装的动力学顺序。