National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
State Key Laboratory of Animal Nutrition, Chinese Academy of Agricultural Science, Beijing 100193, China.
Genes (Basel). 2022 Jan 22;13(2):192. doi: 10.3390/genes13020192.
The role of hexanal in flavor as an indicator of the degree of oxidation of meat products is undeniable. However, the genes and pathways of hexanal formation have not been characterized in detail. In this study, we performed differential gene expression analysis and weighted gene co-expression network analysis (WGCNA) on groups of Tiannong partridge chickens with different relative hexanal content in order to find the genes involved in the formation of hexanal and the specific pathways of hexanal formation. Then we confirmed the relationship of these candidate genes with hexanal using Jingxing Yellow chicken and Wenchang chicken. In this study, WGCNA revealed a module of co-expressed genes that were highly associated with the volatile organic compound hexanal. We also compared transcriptome gene expression data of samples from chicken groups with high and low relative contents of hexanal and identified a total of 651 differentially expressed genes (DEGs). Among them, 356 genes were up regulated, and 295 genes were downregulated. The different biological functions associated with the DEGs, hub genes and hexanal were identified by functional analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations. Among all the hub genes in the significant module identified by WGCNA, more were enriched in the PPAR signaling pathway, the proteasome pathway, etc. Additionally, we found that DEGs and hub genes, including , , , , , , , and , were co-enriched in the peroxisome proliferator-activated receptor (PPAR) signaling pathway, p53 signaling pathway and mitogen-activated protein kinases (MAPK) signaling pathway, etc. Transcriptome results of the Jingxing Yellow chicken population showed that the gene was significantly associated with hexanal and enriched in the PPAR pathway. Our study provides a comprehensive insight into the key genes related to hexanal content, and can be further explored by functional and molecular studies.
己醛作为肉品氧化程度的风味指标作用不可否认。然而，己醛形成的基因和途径尚未得到详细描述。在这项研究中，我们对相对己醛含量不同的天农雉鸡进行差异基因表达分析和加权基因共表达网络分析（WGCNA），以找到参与己醛形成的基因和己醛形成的具体途径。然后，我们使用景星黄鸡和文昌鸡来验证这些候选基因与己醛的关系。在这项研究中，WGCNA 揭示了一个与挥发性有机化合物己醛高度相关的共表达基因模块。我们还比较了高、低相对己醛含量鸡群样本的转录组基因表达数据，共鉴定出 651 个差异表达基因（DEGs）。其中，356 个基因上调，295 个基因下调。通过对 DEGs、枢纽基因和己醛进行京都基因与基因组百科全书（KEGG）注释的功能分析，确定了与 DEGs 相关的不同生物学功能。在 WGCNA 鉴定的显著模块中所有枢纽基因中，更多的基因富集在过氧化物酶体增殖物激活受体（PPAR）信号通路、蛋白酶体通路等。此外，我们发现 DEGs 和枢纽基因，包括 、 、 、 、 、 、 ，在过氧化物酶体增殖物激活受体（PPAR）信号通路、p53 信号通路和丝裂原激活蛋白激酶（MAPK）信号通路等中共同富集。景星黄鸡群体的转录组结果表明，基因与己醛显著相关，并富集在 PPAR 通路中。我们的研究为与己醛含量相关的关键基因提供了全面的见解，并可以通过功能和分子研究进一步探索。
