Achimón Fernanda, Brito Vanessa D, Pizzolitto Romina P, Zygadlo Julio A
Multidisciplinary Institute of Plant Biology (IMBIV-CONICET), National University of Cordoba, Cordoba X5016GCA, Argentina.
Science and Food Technology Institute (ICTA), National University of Cordoba, Cordoba X5016GCA, Argentina.
J Fungi (Basel). 2022 Feb 5;8(2):158. doi: 10.3390/jof8020158.
The aim of the present study was to evaluate the effect of different carbon sources on the hydrocarbon-like volatile organic compounds (VOCs) of strain 7600 through a Principal Component Analysis approach, and to explore their diesel potential by using data from the literature. The fungus was cultivated in GYAM culture medium, and five carbon sources were evaluated: glucose, sucrose, xylose, lactose, and fructose. The VOCs were collected using a close-loop apparatus and identified through GC-MS. The same profile of 81 VOCs was detected with all treatments, but with different relative percentages among carbon sources. The production of branched-chain alkanes (30 compounds) ranged from 25.80% to 38.64%, straight-chain alkanes (12 compounds) from 22.04% to 24.18%, benzene derivatives (12 compounds) from 7.48% to 35.58%, and the biosynthesis of branched-chain alcohols (11 compounds) was from 6.82% to 16.71%, with lower values for the remaining groups of VOCs. Our results show that has the metabolic potential to synthesize diesel-like VOCs. Further research should include the optimization of culture conditions other than carbon sources to increase the production of certain groups of VOCs.
本研究的目的是通过主成分分析方法评估不同碳源对7600菌株类烃挥发性有机化合物(VOCs)的影响,并利用文献数据探索其柴油生产潜力。该真菌在GYAM培养基中培养,评估了五种碳源:葡萄糖、蔗糖、木糖、乳糖和果糖。使用闭环装置收集VOCs,并通过气相色谱 - 质谱联用仪进行鉴定。所有处理均检测到相同的81种VOCs谱图,但不同碳源之间的相对百分比不同。支链烷烃(30种化合物)的产量范围为25.80%至38.64%,直链烷烃(12种化合物)为22.04%至24.18%,苯衍生物(12种化合物)为7.48%至35.58%,支链醇(11种化合物)的生物合成量为6.82%至16.71%,其余VOCs组的值较低。我们的结果表明,该菌株具有合成柴油样VOCs的代谢潜力。进一步的研究应包括优化除碳源以外的培养条件,以增加某些VOCs组的产量。