Institute of Physical Education, Shaanxi Normal University, Xi'an 710119, China.
First Middle School of Shenmu City, Shenmu 719300, China.
Int J Environ Res Public Health. 2022 Feb 18;19(4):2377. doi: 10.3390/ijerph19042377.
Studies have found that the prevalence of chronic fatigue syndrome (CFS) in adolescents has continued to increase over the years, affecting learning and physical health. High school is a critical stage for adolescents to grow and mature. There are inadequate detection and rehabilitation methods for CFS due to an insufficient understanding of the physiological mechanisms of CFS. The purpose of this study was to evaluate the effect and metabolic mechanisms of an aerobic running intervention program for high school students with CFS. Forty-six male high school students with CFS were randomly assigned to the exercise intervention group (EI) and control group (CFS). Twenty-four age- and sex-matched healthy male students were recruited as healthy controls (HCs). The EI group received the aerobic intervention for 12 weeks, three times a week, in 45-min sessions; the CFS group maintained their daily routines as normal. The outcome measures included fatigue symptoms and oxidation levels. Keratin was extracted from the nails of all participants, and the oxidation level was assessed by measuring the content of 3-Nitrotyrosine (3-NT) in the keratin by ultraviolet spectrophotometry. All participants' morning urine was collected to analyze urinary differential metabolites by the GC-MS technique before and after the intervention, and MetaboAnalyst 5.0 was used for pathway analysis. Compared with before the intervention, the fatigue score and 3-NT level in the EI group were significantly decreased after the intervention. The CFS group was screened for 20 differential metabolites involving the disruption of six metabolic pathways, including arginine biosynthesis, glycerolipid metabolism, pentose phosphate pathway, purine metabolism, β-alanine metabolism, and arginine and proline metabolism. After the intervention, 21 differential metabolites were screened, involved in alterations in three metabolic pathways: beta-alanine metabolism, pentose phosphate metabolism, and arginine and proline metabolism. Aerobic exercise was found to lessen fatigue symptoms and oxidative levels in students with CFS, which may be related to the regulation of putrescine (arginine and proline metabolism), 6-Phospho-D-Gluconate (starch and sucrose metabolism pathway), and Pentose (phosphate metabolism pathway).
研究发现，青少年慢性疲劳综合征（CFS）的患病率多年来持续上升，影响学习和身体健康。高中是青少年成长和成熟的关键阶段。由于对 CFS 生理机制的认识不足，对 CFS 的检测和康复方法还不够完善。本研究旨在评估有氧运动干预计划对高中生 CFS 的效果和代谢机制。将 46 名患有 CFS 的男性高中生随机分配到运动干预组（EI）和对照组（CFS）。招募了 24 名年龄和性别匹配的健康男性学生作为健康对照组（HCs）。EI 组接受为期 12 周、每周 3 次、每次 45 分钟的有氧运动干预；CFS 组保持日常作息不变。评估指标包括疲劳症状和氧化水平。从所有参与者的指甲中提取角蛋白，并通过紫外分光光度法测量角蛋白中 3-硝基酪氨酸（3-NT）的含量来评估氧化水平。所有参与者在干预前后均采集清晨尿液，通过 GC-MS 技术分析尿液差异代谢物，并用 MetaboAnalyst 5.0 进行通路分析。与干预前相比，EI 组干预后疲劳评分和 3-NT 水平明显降低。CFS 组筛选出 20 种差异代谢物，涉及 6 种代谢途径的破坏，包括精氨酸生物合成、甘油脂代谢、磷酸戊糖途径、嘌呤代谢、β-丙氨酸代谢和精氨酸和脯氨酸代谢。干预后，筛选出 21 种差异代谢物，涉及三种代谢途径的改变：β-丙氨酸代谢、磷酸戊糖代谢和精氨酸和脯氨酸代谢。有氧运动可减轻 CFS 学生的疲劳症状和氧化水平，这可能与腐胺（精氨酸和脯氨酸代谢）、6-磷酸-D-葡萄糖酸（淀粉和蔗糖代谢途径）和戊糖（磷酸戊糖代谢途径）的调节有关。
