Yanta Christine A, Barta John R, Corbeil Antoine, Menan Hervé, Thivierge Karine, Needle Robert, Morshed Muhammad, Dixon Brent R, Wasmuth James D, Guy Rebecca A
National Microbiology Laboratory at Guelph, Division of Enteric Diseases, Public Health Agency of Canada, Guelph, ON N1G 3W4, Canada.
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.
Microorganisms. 2022 Feb 15;10(2):447. doi: 10.3390/microorganisms10020447.
is an emerging foodborne parasite that causes cyclosporiasis, an enteric disease of humans. Domestically acquired outbreaks have been reported in Canada every spring or summer since 2013. To date, investigations into the potential sources of infection have relied solely on epidemiological data. To supplement the epidemiological data with genetic information, we genotyped 169 Canadian cyclosporiasis cases from stool specimens collected from 2010 to 2021 using an existing eight-marker targeted amplicon deep (TADS) scheme specific to as previously described by the US Centers for Disease Control and Prevention (CDC). This is the first study to genotype Canadian isolates, and it focuses on evaluating the genotyping performance and genetic clustering. Genotyping information was successfully collected with at least part of one of the markers in the TADS assay for 97.9% of specimens, and 81.1% of cyclosporiasis cases met the minimum requirements to genetically cluster into 20 groups. The performance of the scheme suggests that examining cyclosporiasis cases genetically will be a valuable tool for supplementing epidemiological outbreak investigations and to minimize further infections. Further research is required to expand the number of discriminatory markers to improve genetic clustering.
是一种新兴的食源性病原体，可导致环孢子虫病，这是一种人类肠道疾病。自2013年以来，加拿大每年春季或夏季都会报告国内感染爆发情况。迄今为止，对潜在感染源的调查仅依赖于流行病学数据。为了用基因信息补充流行病学数据，我们使用美国疾病控制与预防中心（CDC）先前描述的针对的现有八标记靶向扩增子深度（TADS）方案，对2010年至2021年收集的粪便标本中的169例加拿大环孢子虫病病例进行了基因分型。这是首次对加拿大分离株进行基因分型的研究，重点是评估基因分型性能和基因聚类情况。在TADS检测中，97.9%的标本成功收集到至少一个标记的部分基因分型信息，81.1%的环孢子虫病病例符合基因聚类为20组的最低要求。该方案的性能表明，对环孢子虫病病例进行基因检测将是补充流行病学爆发调查和尽量减少进一步感染的宝贵工具。需要进一步研究以增加鉴别标记的数量，以改善基因聚类。
