来自副产物的提取物的体内抗炎作用、抗氧化活性和多酚含量。

In Vivo Anti-Inflammatory Effect, Antioxidant Activity, and Polyphenolic Content of Extracts from By-Products.

机构信息

Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A. C. Subsede Sureste, Tablaje 31264 km, 5.5 Carretera Sierra Papacal-Chuburna Puerto, Parque Científico Tecnológico de Yucatán, Mérida C.P. 97302, Mexico.

Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col Chamilpa, Cuernavaca C.P. 62209, Mexico.

出版信息

Molecules. 2022 Feb 16;27(4):1323. doi: 10.3390/molecules27041323.

DOI:10.3390/molecules27041323

PMID:35209112

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8880488/

Abstract

By-products of Jacq., var Jaguar could be a source of bioactive compounds. Therefore, we evaluated the anti-inflammatory effect, antioxidant activity, and their relationship with the polyphenol content of extracts of habanero pepper by-products obtained from plants grown on black or red soils of Yucatán, Mexico. Moreover, the impact of the type of extraction on their activities was evaluated. The dry by-product extracts were obtained by maceration (ME), Soxhlet (SOX), and supercritical fluid extraction (SFE). Afterward, the in vivo anti-inflammatory effect (TPA-induced ear inflammation) and the in vitro antioxidant activity (ABTS) were evaluated. Finally, the polyphenolic content was quantified by Ultra-Performance Liquid Chromatography (UPLC), and its correlation with both bioactivities was analyzed. The results showed that the SFE extract of stems of plants grown on red soil yielded the highest anti-inflammatory effect (66.1 ± 3.1%), while the extracts obtained by ME and SOX had the highest antioxidant activity (2.80 ± 0.0052 mM Trolox equivalent) and polyphenol content (3280 ± 15.59 mg·100 g dry basis), respectively. A negative correlation between the anti-inflammatory effect, the antioxidant activity, and the polyphenolic content was found. Overall, the present study proposed by-products as a valuable source of compounds with anti-inflammatory effect and antioxidant activity.

摘要

由 Jacq. 产生的副产物，即 Jaguar 可能是生物活性化合物的来源。因此，我们评估了来自墨西哥尤卡坦黑土或红土种植的植物的 habanero 辣椒副产物提取物的抗炎作用、抗氧化活性及其与多酚含量的关系。此外，还评估了提取类型对其活性的影响。通过浸渍（ME）、索氏提取（SOX）和超临界流体提取（SFE）获得干燥副产物提取物。然后，评估体内抗炎作用（TPA 诱导的耳炎）和体外抗氧化活性（ABTS）。最后，通过超高效液相色谱（UPLC）定量测定多酚含量，并分析其与两种生物活性的相关性。结果表明，生长在红土上的植物茎的 SFE 提取物具有最高的抗炎作用（66.1 ± 3.1%），而 ME 和 SOX 提取物具有最高的抗氧化活性（2.80 ± 0.0052 mM Trolox 当量）和多酚含量（3280 ± 15.59 mg·100 g 干基）。抗炎作用、抗氧化活性和多酚含量之间存在负相关关系。总的来说，本研究提出副产物是具有抗炎作用和抗氧化活性的化合物的有价值来源。