College of Agriculture, Guangxi University, Nanning, 530004, China.
College of Chemistry and Environmental Engineering, Hanjiang Normal University, Shiyan, 442000, China.
BMC Genomics. 2022 Feb 24;23(1):161. doi: 10.1186/s12864-022-08330-0.
Paris polyphylla is a herb widely used in traditional Chinese medicine to treat various diseases. Stem rot diseases seriously affected the yield of P. polyphylla in subtropical areas of China. Therefore, cost-effective, chemical-free, eco-friendly strategies to control stem rot on P. polyphylla are valuable and urgently needed.
In this paper, we reported the biocontrol efficiency of Paenibacillus peoriae HJ-2 and its complete genome sequence. Strain HJ-2 could serve as a potential biocontrol agent against stem rot on P. polyphylla in the greenhouse and field. The genome of HJ-2 consists of a single 6,001,192 bp chromosome with an average GC content of 45% and 5,237 predicted protein coding genes, 39 rRNAs and 108 tRNAs. The phylogenetic tree indicated that HJ-2 is most closely related to P. peoriae IBSD35. Functional analysis of genome revealed numerous genes/gene clusters involved in plant colonization, biofilm formation, plant growth promotion, antibiotic and resistance inducers synthesis. Moreover, metabolic pathways that potentially contribute to biocontrol mechanisms were identified.
This study revealed that P. peoriae HJ-2 could serve as a potential BCA against stem rot on P. polyphylla. Based on genome analysis, the genome of HJ-2 contains more than 70 genes and 12 putative gene clusters related to secondary metabolites, which have previously been described as being involved in chemotaxis motility, biofilm formation, growth promotion, antifungal activity and resistance inducers biosynthesis. Compared with other strains, variation in the genes/gene clusters may lead to different antimicrobial spectra and biocontrol efficacies.
重楼是一种广泛应用于传统中药治疗各种疾病的草本植物。茎腐病严重影响了中国亚热带地区重楼的产量。因此，寻找经济高效、无化学物质、环保的策略来控制重楼的茎腐病是非常有价值且迫切需要的。
本文报道了佩诺氏芽孢杆菌 HJ-2 的生防效率及其全基因组序列。HJ-2 菌株可作为温室和田间防治重楼茎腐病的潜在生防剂。HJ-2 的基因组由一个 6,001,192 bp 的单染色体组成，平均 GC 含量为 45%，预测有 5,237 个蛋白编码基因、39 个 rRNA 和 108 个 tRNA。系统发育树表明 HJ-2 与佩诺氏芽孢杆菌 IBSD35 最为密切相关。基因组功能分析显示，大量与植物定殖、生物膜形成、植物生长促进、抗生素和抗性诱导物合成有关的基因/基因簇。此外，还鉴定了可能有助于生防机制的代谢途径。
本研究表明，佩诺氏芽孢杆菌 HJ-2 可作为防治重楼茎腐病的潜在生防菌。基于基因组分析，HJ-2 基因组中含有 70 多个基因和 12 个可能与次生代谢物有关的基因簇，这些基因簇先前被描述为参与趋化运动性、生物膜形成、生长促进、抗真菌活性和抗性诱导物合成。与其他菌株相比，基因/基因簇的变异可能导致不同的抗菌谱和生防效果。
