Hamman Naasika, Ramburrun Poornima, Dube Admire
Infectious Disease Nanomedicine Research Group, School of Pharmacy, University of the Western Cape, Bellville 7535, South Africa.
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2193, South Africa.
Pharmaceutics. 2024 Mar 25;16(4):450. doi: 10.3390/pharmaceutics16040450.
The disruption of periodontal biofilms and prevailing antimicrobial resistance issues continue to pose a great challenge to the treatment of periodontitis. Here, we report on selenium nanoparticles (SeNPs) as a treatment alternative for periodontitis by determining their antibiofilm activity against biofilms and the potential role of particle size in disrupting biofilms. SeNPs were synthesised via a reduction reaction. Various physicochemical characterisations were conducted on the NPs, including size and shape. The microbroth dilution method was used to conduct the biofilm and antibiofilm assay against , which was analysed by absorbance. SeNPs displayed hydrodynamic sizes as low as 46 ± 4 nm at a volume ratio of 1:5 (sodium selenite/ascorbic acid) with good monodispersity and stability. Hydrodynamic sizes of SeNPs after resuspension in tryptic soy broth supplemented with 2.5% sucrose (TSB + 2.5% suc.) and incubated at 37 °C for 24 h, ranged from 112 to 263 nm, while the zeta potential values increased to greater than -11 mV. The biofilm assay indicated that are weakly adherent, bordering on moderately adherent biofilm producers. The minimum biofilm inhibitory concentration (MBIC) was identified at 500 µg/mL. At a 1000 µg/mL concentration, SeNPs were able to inhibit biofilms up to 99.87 ± 2.41% at a volume ratio of 1:1. No correlation was found between antibiofilm activity and particle size; however, antibiofilm activity was proven to be concentration-dependant. SeNPs demonstrate antibiofilm activity and may be useful for further development in treating periodontitis.
牙周生物膜的破坏以及普遍存在的抗菌耐药性问题，仍然对牙周炎的治疗构成巨大挑战。在此，我们报告了硒纳米颗粒（SeNPs）作为牙周炎治疗替代方案的研究，通过确定其对生物膜的抗生物膜活性以及颗粒大小在破坏生物膜中的潜在作用。SeNPs通过还原反应合成。对这些纳米颗粒进行了各种物理化学表征，包括大小和形状。采用微量肉汤稀释法对 进行生物膜和抗生物膜测定，并通过吸光度进行分析。在1:5（亚硒酸钠/抗坏血酸）的体积比下，SeNPs显示出低至46±4 nm的流体动力学尺寸，具有良好的单分散性和稳定性。将SeNPs重新悬浮在补充有2.5%蔗糖的胰蛋白胨大豆肉汤（TSB + 2.5% suc.）中，并在37°C下孵育24小时后，其流体动力学尺寸范围为112至263 nm，而zeta电位值增加到大于 -11 mV。生物膜测定表明 附着较弱，接近于中度附着生物膜产生菌。最低生物膜抑制浓度（MBIC）确定为500 µg/mL。在1000 µg/mL的浓度下，在1:1的体积比下，SeNPs能够抑制 生物膜高达99.87±2.41%。未发现抗生物膜活性与颗粒大小之间存在相关性；然而，抗生物膜活性被证明是浓度依赖性的。SeNPs表现出抗生物膜活性，可能有助于牙周炎治疗的进一步发展。
