Department of Genetics and Plant Breeding, Faculty of Agronomy, Horticulture and Bioengineering, Poznań University of Life Sciences, 11 Dojazd Str, 60-632 Poznań, Poland.
Biomol Concepts. 2022 Feb 22;13(1):1-9. doi: 10.1515/bmc-2022-0002.
Wheat leaf rust, caused by fungal pathogen Erikss, annually contributes to production losses as high as 40% in susceptible varieties and remains as one of the most damaging diseases of wheat worldwide. Currently, one of the major challenges of wheat geneticists and breeders is to accumulate major genes for durability of rust resistance called "slow rusting" genes using marker-assisted selection (MAS). Until now, eight genes (, , , , , , , and ) conferring resistance against multiple fungal pathogens have been identified in wheat gene pool and the molecular markers were developed for them. In MAS practice, it is a common problem that cultivars exhibiting desirable marker genotypes may not necessarily have the targeted genes or alleles and vice versa, which is known as "false positives." The aim of this study was to compare the available four markers: , , , and markers (not published yet), for the identification of the loci in 73 genotypes of wheat, which were reported as sources of various "slow rusting" genes, including 60 with confirmed gene, reported in the literature. This research revealed that together with is most suitable for the identification of resistance allele of the gene; however, there is a need to clone the loci to identify and verify the allelic variation of the gene and the function.
小麦叶锈病由真菌病原体 Erikss 引起，在易感染品种中，每年造成的损失高达 40%，是全球小麦最具破坏性的疾病之一。目前，小麦遗传学家和培育者面临的主要挑战之一是利用标记辅助选择（MAS）积累具有耐久性的锈病抗性的主要基因，称为“慢锈性”基因。到目前为止，已经在小麦基因库中鉴定出了八个基因（、、、、、、和），它们对多种真菌病原体具有抗性，并且已经为它们开发了分子标记。在 MAS 实践中，一个常见的问题是，表现出理想标记基因型的品种不一定具有目标基因或等位基因，反之亦然，这被称为“假阳性”。本研究的目的是比较现有的四个标记：、、和 标记（尚未发表），用于鉴定 73 个小麦基因型中的 基因座，这些基因型被报道为各种“慢锈性”基因的来源，包括 60 个经文献报道具有确认的 基因。这项研究表明， 与 一起最适合鉴定 基因的抗性等位基因；然而，需要克隆 基因座来鉴定和验证基因的等位基因变异和功能。
