Lee Chung-Yuan, Hsin Min-Chieh, Chen Pei-Ni, Lin Chiao-Wen, Wang Po-Hui, Yang Shun-Fa, Hsiao Yi-Hsuan
Department of Obstetrics and Gynecology, Chiayi Chang Gung Memorial Hospital, Chiayi 613, Taiwan.
Department of Nursing, Chang Gung University of Science and Technology, Chiayi Campus, Chiayi 613, Taiwan.
Pharmaceutics. 2022 Feb 5;14(2):365. doi: 10.3390/pharmaceutics14020365.
Arctiin, a lignan glycoside, is isolated from L. The anticancer effects of arctiin have been demonstrated in several studies. However, no research has been conducted on the anti-migration effect of arctiin in cervical cancer cells. The present study examined the effects of arctiin on cervical cancer cells and investigated the possible molecular mechanism. We demonstrated that arctiin exhibited low cytotoxicity and significantly inhibited cell migration and invasion in human cervical cancer cells. The S100A4 protein expression and mRNA levels were significantly reduced in HeLa and SiHa cells with arctiin treatment. Furthermore, silencing S100A4 by using small interfering RNA reduced cell migration, while overexpression of S100A4 mitigated the migration inhibition imposed by arctiin in cervical cancer cells. Western blotting revealed that arctiin significantly reduced phosphoinositide 3-kinase (PI3K) and phosphorylation of Akt in cervical cancer cells. Moreover, selective Akt induction by an Akt activator, SC-79, reverted cervical cancer cell migration and S100A4 protein expression, which were reduced in response to arctiin. Taken together, these results suggest that arctiin inhibits cervical cancer cell migration and invasion through suppression of S100A4 and the PI3K/Akt pathway.
牛蒡子苷，一种木脂素糖苷，从牛蒡中分离得到。多项研究已证实牛蒡子苷具有抗癌作用。然而，尚未有关于牛蒡子苷对宫颈癌细胞抗迁移作用的研究。本研究检测了牛蒡子苷对宫颈癌细胞的影响，并探讨了其可能的分子机制。我们发现牛蒡子苷对人宫颈癌细胞具有低细胞毒性，并能显著抑制细胞迁移和侵袭。经牛蒡子苷处理后，HeLa和SiHa细胞中S100A4蛋白表达和mRNA水平显著降低。此外，使用小干扰RNA沉默S100A4可减少细胞迁移，而S100A4的过表达则减轻了牛蒡子苷对宫颈癌细胞迁移的抑制作用。蛋白质印迹法显示，牛蒡子苷可显著降低宫颈癌细胞中磷酸肌醇3激酶（PI3K）和Akt的磷酸化水平。此外，Akt激活剂SC-79选择性诱导Akt可逆转因牛蒡子苷处理而降低的宫颈癌细胞迁移和S100A4蛋白表达。综上所述，这些结果表明牛蒡子苷通过抑制S100A4和PI3K/Akt信号通路来抑制宫颈癌细胞的迁移和侵袭。
