Department of Chemistry, Ayatollah Amoli Branch, Islamic Azad University, Amol, 46131, Iran.
Univ. Lille, CNRS, Centrale Lille, ISEN, Univ. Valenciennes, UMR 8520-IEMN, 59000, Lille, France.
Anal Bioanal Chem. 2019 Mar;411(8):1509-1516. doi: 10.1007/s00216-019-01611-w. Epub 2019 Feb 9.
Doxorubicin (DOX), an anthracycline molecule, is currently one of the most widely used anticancer drugs in clinics. Systematic treatment of patients with DOX is known to be accompanied by several unpleasant side effects due to the toxicity of the drug. Thus, monitoring of DOX concentration in serum samples has become increasingly important to avoid side effects and ensure therapeutic efficiency. In this study, we discuss the construction of a disposable electrochemical sensor for the direct monitoring of DOX in clinical blood samples. The sensor is based on coating a gold electrode in a flexible integrated electrode construct formed on polyimide sheets using photolithography, with nitrogen-doped reduced graphene oxide (N-rGO) suspended in chitosan. Under optimized conditions, a linear relationship between the oxidative peak current and the concentration of DOX in the range of 0.010-15 μM with a detection limit of 10 nM could be achieved. The sensor was adapted to monitor DOX in serum samples of patients under anticancer treatment. Graphical abstract.
阿霉素（DOX）是一种蒽环类分子，目前是临床中应用最广泛的抗癌药物之一。系统治疗 DOX 的患者已知会因药物毒性而产生几种不良副作用。因此，监测血清样本中的 DOX 浓度对于避免副作用和确保治疗效率变得越来越重要。在这项研究中，我们讨论了一种用于直接监测临床血液样本中 DOX 的一次性电化学传感器的构建。该传感器基于在聚酰亚胺片上使用光刻形成的柔性集成电极结构中的金电极上涂覆悬浮在壳聚糖中的氮掺杂还原氧化石墨烯（N-rGO）。在优化条件下，在 0.010-15 μM 的范围内，氧化峰电流与 DOX 浓度之间呈线性关系，检测限为 10 nM。该传感器适用于监测接受抗癌治疗的患者的血清样本中的 DOX。
