Ali Asmaa R, Anani Haneya A A, Selim Fatma M
Department of Medical Microbiology and Immunology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
Iran J Microbiol. 2021 Dec;13(6):848-861. doi: 10.18502/ijm.v13i6.8090.
Silver nanoparticles (AgNPs) have been found to have multiple uses as antibacterial, antifungal and anti-biofilm agents because of their biological activities and safety. The present study was aimed to analyze the antimicrobial and anti-biofilm activities as well as the cytotoxic effect of AgNPs against different human pathogens.
AgNPs were synthesized using cell free supernatants of (ATCC 25922), (ATCC 19433), (ATCC 27856), (ATCC 13047) and strain, then were analyzed using UV/Vis Spectral Analysis, Transmission electron microscopy (TEM). Scanning Electron Microscope (SEM) and Energy Dispersive-X-ray Spectroscopy (EDX) analysis. Antimicrobial activities of biosynthesized AgNPs were assessed with selected antimicrobial agents against multidrug resistant bacteria and candida. Anti-biofilm and cytotoxicity assays of these biosynthesized AgNPs were also done.
The synthesis of AgNPs were confirmed through observed color change and monitoring UV-Vis spectrum which showed homogeneous (little agglomeration) distribution of silver nanoparticles. TEM and SEM have shown that the particle size ranged from 13 to 34 (nm) with spherical shape and a high signal with EDX analysis. Antibacterial and antifungal efficacy of antibiotics and fluconazole were increased in combination with biosynthesized AgNPs against resistant bacteria and candida. Significant reduction in biofilm formation was found better with AgNPs against biofilm forming bacteria.
has the best effect towards synthesizing AgNPs, for antimicrobial activities against resistant bacteria and candida, in addition to anti-biofilm activities against biofilm forming and and the safest cytotoxicity effect on (MRC-5) cell line.
由于银纳米颗粒（AgNPs）具有生物活性和安全性，已发现其作为抗菌、抗真菌和抗生物膜剂有多种用途。本研究旨在分析AgNPs对不同人类病原体的抗菌和抗生物膜活性以及细胞毒性作用。
使用金黄色葡萄球菌（ATCC 25922）、枯草芽孢杆菌（ATCC 19433）、大肠杆菌（ATCC 27856）、铜绿假单胞菌（ATCC 13047）和白色念珠菌菌株的无细胞上清液合成AgNPs，然后使用紫外/可见光谱分析、透射电子显微镜（TEM）、扫描电子显微镜（SEM）和能量色散X射线光谱（EDX）分析进行分析。用选定的抗菌剂评估生物合成的AgNPs对多重耐药细菌和念珠菌的抗菌活性。还对这些生物合成的AgNPs进行了抗生物膜和细胞毒性试验。
通过观察颜色变化和监测紫外-可见光谱证实了AgNPs的合成，该光谱显示银纳米颗粒分布均匀（团聚少）。TEM和SEM表明，粒径范围为13至34纳米，呈球形，EDX分析显示信号强。抗生素和氟康唑与生物合成的AgNPs联合使用时，对耐药细菌和念珠菌的抗菌和抗真菌效果增强。发现金黄色葡萄球菌AgNPs对形成生物膜的细菌的生物膜形成有更显著的减少作用。
金黄色葡萄球菌对合成AgNPs的效果最佳，对耐药细菌和念珠菌具有抗菌活性，此外对形成生物膜的金黄色葡萄球菌和白色念珠菌具有抗生物膜活性，并且对人胚肺成纤维细胞（MRC-5）细胞系的细胞毒性作用最安全。
