Mehdizadeh Maryam, Sheikhpour Mojgan, Salahshourifar Iman, Siadat Seyed Davar, Saffarian Parvaneh
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Iran J Public Health. 2021 Nov;50(11):2292-2301. doi: 10.18502/ijph.v50i11.7585.
We aimed to prepare a nanofluid, containing -MWCNTs, and investigate the antibacterial efficacy of -MWCNTs+ ciprofloxacin (cip) on by evaluating the virulence gene expression.
This study was carried out from 2019 to 2020, in the Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran. The nanofluid containing antibiotic and -MWCNTs were prepared by the ultrasonic method. The minimum inhibitory concentrations (MICs) of ciprofloxacin and -MWCNTs were determined using the broth micro dilution MIC tests. For examining the antibacterial effects, the expression level of virulence genes, under the influence of -MWCNTs, was evaluated by a real-time PCR.
The effect of 8 μg/ml ciprofloxacin + 400 μg/ml -MWCNTs, completely inhibited the growth of the resistant isolate of , while, in the ATCC 700,603 isolate, 2 μg/ml ciprofloxacin with 100 μg/ml MWCNT could inhibit a bacterial growth. In the resistant clinical isolate after -MWCNT+cip treatment, the expression of , , and genes was significantly downregulated, compared to the ciprofloxacin treatment, and upregulated, compared to the negative control. For the ATCC 700,603 isolate treated with -MWCNT+cip, the expression of , and virulence genes showed upregulation, compared to the negative control and downregulated in comparison with the ciprofloxacin treatment.
Simultaneous treatment of resistant isolate of with -MWCNTs +antibiotic could improve the effectiveness of antibiotic at lower doses, due to the reduced expression of virulence genes in comparison with antibiotic treatment, besides the increased cell wall permeability to antibiotics.
我们旨在制备一种含有多壁碳纳米管（MWCNTs）的纳米流体，并通过评估毒力基因表达来研究MWCNTs+环丙沙星（cip）对[具体细菌名称未给出]的抗菌效果。
本研究于2019年至2020年在伊朗巴斯德研究所的分枝杆菌学与肺部研究部进行。通过超声法制备含有抗生素和MWCNTs的纳米流体。使用肉汤微量稀释MIC试验测定环丙沙星和MWCNTs的最低抑菌浓度（MICs）。为了检测抗菌效果，通过实时PCR评估MWCNTs影响下毒力基因的表达水平。
8μg/ml环丙沙星 + 400μg/ml MWCNTs的组合完全抑制了[具体细菌名称未给出]耐药菌株的生长，而在ATCC 700,603菌株中，2μg/ml环丙沙星与100μg/ml MWCNT可抑制细菌生长。在MWCNT+cip治疗后的耐药[具体细菌名称未给出]临床分离株中，与环丙沙星治疗相比，[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]基因的表达显著下调，与阴性对照相比则上调。对于用MWCNT+cip处理的ATCC 700,603菌株，与阴性对照相比，[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]毒力基因的表达上调，与环丙沙星治疗相比则下调。
用MWCNTs +抗生素同时治疗[具体细菌名称未给出]耐药分离株，与抗生素治疗相比，由于毒力基因表达降低以及细胞壁对抗生素的通透性增加，可在较低剂量下提高抗生素的有效性。
