Tirol Kliniken GmbH, Zentralinstitut für Bluttransfusion und Immunologische Abteilung (ZIB), Innsbruck, Austria.
Mallinckrodt Pharmaceuticals, 3 Lotus Park, The Causeway, Middlesex, UK.
J Clin Apher. 2022 Aug;37(4):332-339. doi: 10.1002/jca.21974. Epub 2022 Feb 28.
Extracorporeal photopheresis (ECP) is a blood-based therapeutic procedure increasingly used for modulation of immune dysregulation in various underlying disease settings. The aim of this study was to compare the procedure times and blood collection efficiencies between the two approaches currently utilized in European centers: the integrated versus the multistep nonintegrated procedures.
A retrospective data analysis was conducted, comparing treatment data from patients who received ECP therapy at the Central Institute for Blood Transfusion & Department of Immunology (ZIB) of the Tirol Kliniken GmbH, where the integrated and multistep nonintegrated procedures are routinely used in an approximated setup.
During the observation period, a total of 15 patients who were treated with alternating systems on 2 consecutive days were identified. This allowed treatment pair comparisons with minimal interpatient variabilities, similar to a cross-over design even though analyzed retrospectively. Total average procedure times with the integrated system were 99.3 vs 122.0 minutes with the multistep nonintegrated procedures, respectively. Significant differences were observed for all steps of the ECP procedure: (a) time for buffy coat collection, 66.5 vs 74.7; (b) handling/transfer, 2.8 vs 18.7; (c) irradiation, 20.3 vs 11.7; and (d) reinfusion/handling time, 9.6 vs 16.3 minutes. The calculated collection throughput was 7.79 mL/min for the integrated and 7.84 mL/min for the multistep nonintegrated procedures, and with a white blood cell (WBC) collection efficiency of 34.2% and 21.0%, respectively.
The data presented in this study show a significant shorter overall procedure time and higher WBC collection efficiency for the integrated ECP system.
体外光分离术（ECP）是一种基于血液的治疗方法，越来越多地用于调节各种基础疾病中的免疫失调。本研究的目的是比较目前欧洲中心使用的两种方法的程序时间和血液采集效率：集成方法与非集成的多步方法。
对在蒂罗尔临床中心输血研究所和免疫学部（ZIB）接受 ECP 治疗的患者进行回顾性数据分析，该中心常规使用集成和非集成方法。
在观察期间，确定了 15 名患者，他们在连续两天内接受了交替系统的治疗。这允许进行治疗对比较，患者之间的差异最小，类似于交叉设计，即使是回顾性分析。使用集成系统的总平均程序时间为 99.3 分钟，而非集成的多步方法为 122.0 分钟。在 ECP 程序的所有步骤中均观察到显著差异：（a）采集白细胞层的时间，分别为 66.5 分钟和 74.7 分钟；（b）处理/转移时间，分别为 2.8 分钟和 18.7 分钟；（c）照射时间，分别为 20.3 分钟和 11.7 分钟；（d）再输注/处理时间，分别为 9.6 分钟和 16.3 分钟。集成系统的采集通量为 7.79 mL/min，非集成的多步系统为 7.84 mL/min，白细胞（WBC）采集效率分别为 34.2%和 21.0%。
本研究提供的数据表明，集成 ECP 系统的整体程序时间更短，WBC 采集效率更高。
