在存在粪便大肠菌群的情况下，对用于直接检测产肠毒素大肠杆菌的DNA过滤检测法进行评估和优化。
Evaluation and optimization of the DNA filter assay for direct detection of enterotoxigenic Escherichia coli in the presence of stool coliforms.
作者信息
Yam W C, Lung M L, Ng M H
出版信息
J Clin Microbiol. 1986 Jul;24(1):149-51. doi: 10.1128/jcm.24.1.149-151.1986.
Abstract
Direct detection of enterotoxigenic Escherichia coli (ETEC) in mixed cultures by DNA filter assay was affected by the presence of other bacteria in the cultures. By shortening incubation to 6 h, bacterial overgrowth was minimized, allowing direct detection of ETEC in stools or mixed cultures initially containing 0.2 to 3% ETEC.
摘要
通过DNA滤膜分析直接检测混合培养物中的产肠毒素大肠杆菌（ETEC）会受到培养物中其他细菌存在的影响。通过将培养时间缩短至6小时，可将细菌过度生长降至最低，从而能够直接检测最初含有0.2%至3% ETEC的粪便或混合培养物中的ETEC。
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