用于检测霍乱毒素和大肠杆菌不耐热毒素的乳胶凝集试验的评估与优化
Evaluation and optimization of a latex agglutination assay for detection of cholera toxin and Escherichia coli heat-labile toxin.
作者信息
Yam W C, Lung M L, Ng M H
机构信息
Department of Microbiology, University of Hong Kong.
出版信息
J Clin Microbiol. 1992 Sep;30(9):2518-20. doi: 10.1128/jcm.30.9.2518-2520.1992.
Abstract
The effectiveness of a latex agglutination assay kit for the detection of Escherichia coli heat-labile toxin and cholera toxin was determined for the identification of natural isolates of the corresponding enteric pathogens in Southeast Asia. By selection of the appropriate culture media, the sensitivity of the assay was improved from 90.6% (for the detection of heat-labile toxin) and 75% (for the detection of cholera toxin) to 100%, and the results were confirmed with bioassays and DNA hybridization assays for both clinical and environmental isolates.
摘要
一种用于检测大肠杆菌不耐热毒素和霍乱毒素的乳胶凝集试验试剂盒，被用于鉴定东南亚地区相应肠道病原体的天然分离株，以此来确定该试剂盒的有效性。通过选择合适的培养基，该检测方法的灵敏度从90.6%（用于检测不耐热毒素）和75%（用于检测霍乱毒素）提高到了100%，并且临床和环境分离株的检测结果均通过生物测定法和DNA杂交测定法得到了证实。
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