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J Clin Microbiol. 1992 Sep;30(9):2518-20. doi: 10.1128/jcm.30.9.2518-2520.1992.
2
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Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.用于检测产肠毒素大肠杆菌热不稳定肠毒素的改良埃莱克试验。
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Nucleotide sequence analysis of the A2 and B subunits of Vibrio cholerae enterotoxin.霍乱弧菌肠毒素A2和B亚基的核苷酸序列分析
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Automatic and manual latex agglutination tests for measurement of cholera toxin and heat-labile enterotoxin of Escherichia coli.用于检测霍乱毒素和大肠杆菌不耐热肠毒素的自动和手动乳胶凝集试验。
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Evaluation and optimization of the DNA filter assay for direct detection of enterotoxigenic Escherichia coli in the presence of stool coliforms.在存在粪便大肠菌群的情况下，对用于直接检测产肠毒素大肠杆菌的DNA过滤检测法进行评估和优化。
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用于检测霍乱毒素和大肠杆菌不耐热毒素的乳胶凝集试验的评估与优化

Evaluation and optimization of a latex agglutination assay for detection of cholera toxin and Escherichia coli heat-labile toxin.

作者信息

Yam W C, Lung M L, Ng M H

机构信息

Department of Microbiology, University of Hong Kong.

出版信息

J Clin Microbiol. 1992 Sep;30(9):2518-20. doi: 10.1128/jcm.30.9.2518-2520.1992.

DOI:10.1128/jcm.30.9.2518-2520.1992
PMID:1401032
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265542/
Abstract

The effectiveness of a latex agglutination assay kit for the detection of Escherichia coli heat-labile toxin and cholera toxin was determined for the identification of natural isolates of the corresponding enteric pathogens in Southeast Asia. By selection of the appropriate culture media, the sensitivity of the assay was improved from 90.6% (for the detection of heat-labile toxin) and 75% (for the detection of cholera toxin) to 100%, and the results were confirmed with bioassays and DNA hybridization assays for both clinical and environmental isolates.

摘要

一种用于检测大肠杆菌不耐热毒素和霍乱毒素的乳胶凝集试验试剂盒，被用于鉴定东南亚地区相应肠道病原体的天然分离株，以此来确定该试剂盒的有效性。通过选择合适的培养基，该检测方法的灵敏度从90.6%（用于检测不耐热毒素）和75%（用于检测霍乱毒素）提高到了100%，并且临床和环境分离株的检测结果均通过生物测定法和DNA杂交测定法得到了证实。