Leong Pui Yee, Tan Wei Qi, Choo Wee Sim
School of Science, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia.
FEMS Microbiol Lett. 2025 Jan 10;372. doi: 10.1093/femsle/fnaf020.
Antibiotic resistance and the persistence of sessile cells within biofilms complicate the eradication of biofilm-related infections using conventional antibiotics. This highlights the necessity for alternate therapy methods. The objective of this study was to investigate the biofilm destruction activity of α-tocopherol against Staphylococcus aureus, Proteus mirabilis, and Pseudomonas aeruginosa on polystyrene. α-Tocopherol showed significant biofilm destruction activity on the pre-formed biofilms of S. aureus (45%-46%), Pr. mirabilis (42%-54%), and Ps. aeruginosa (28%). Resazurin assay showed that α-tocopherol disrupted all bacterial biofilms without interfering with their cell viability. Scanning electron microscope images showed lower bacterial cell count and less compacted cell aggregates on polystyrene surfaces after treatment with α-tocopherol. This study demonstrated the biofilm destruction activity of α-tocopherol against S. aureus, Pr. mirabilis, and Ps. aeruginosa. α-Tocopherol could potentially be used to decrease biofilm-associated infections of these bacteria.
抗生素耐药性以及生物膜内固着细胞的持续存在，使得使用传统抗生素根除与生物膜相关的感染变得复杂。这凸显了采用替代治疗方法的必要性。本研究的目的是调查α-生育酚对聚苯乙烯上金黄色葡萄球菌、奇异变形杆菌和铜绿假单胞菌的生物膜破坏活性。α-生育酚对金黄色葡萄球菌（45%-46%）、奇异变形杆菌（42%-54%）和铜绿假单胞菌（28%）预先形成的生物膜显示出显著的生物膜破坏活性。刃天青试验表明，α-生育酚可破坏所有细菌生物膜，而不影响其细胞活力。扫描电子显微镜图像显示，用α-生育酚处理后，聚苯乙烯表面的细菌细胞数量减少，细胞聚集体不那么紧密。本研究证明了α-生育酚对金黄色葡萄球菌、奇异变形杆菌和铜绿假单胞菌的生物膜破坏活性。α-生育酚有可能用于减少这些细菌的生物膜相关感染。
