Early Infancy Special Care Unit, Department of Pediatric care, Regina Margherita Children's Hospital, AOU, Città della Salute e della Scienza di Torino, 10126 Torino, Italy
Department of Public Health and Pediatric Sciences, Pediatric Laboratory, University of Turin, Medical School, 10126 Turin, Italy.
BMJ Open. 2022 Feb 28;12(2):e053323. doi: 10.1136/bmjopen-2021-053323.
The aim of this study was to measure interferon gamma (IFN-γ) and indoleamine 2,3-dioxygenase 1 (IDO1) values in the White blood cells of infants during respiratory tract infections and to compare these with healthy age-matched controls.
This was a prospective, observational case-control study conducted in 2019-2020.
The study took place at Regina Margherita Children's Hospital, Turin, Italy.
The study comprised 63 infants, including 26 patients hospitalised for bronchiolitis due to a respiratory syncytial virus (RSV) infection and 37 age-matched controls. The inclusion criteria included a positive RSV test for an infant with bronchiolitis.
We collected peripheral blood and measured the relative quantification of messenger RNA (mRNA) expression of IFN-γ and IDO1 with TaqMan real-time PCR amplification. The data were collected on the first day of admission.
The mean age of the 26 patients with RSV bronchiolitis (53.8% female) was 85 (9-346) days when they were admitted to the hospital. Their mean gestational age at birth was 38 weeks and their mean birth weight was 3100 (2780-3730) g. The expression of IFN-γ was significantly reduced in patients with bronchiolitis RSV compared with healthy controls (p=0.0132). However, there was no significant difference between the two groups when the IDO1 mRNA expression values in their WCC were measured (p=0.0642).
Our findings did not clarify whether IDO1 expression was related to the early stage of the disease or to the young age of the infants. The data provide evidence that IFN-γ was significantly reduced in infants with bronchiolitis due to RSV, compared with age-matched healthy controls, but the IDO1 was not different. New investigations that focus on subjects infected with RSV at different stages of infancy would help to clarify whether IDO1 expression can be related to age.
本研究旨在测量呼吸道感染患儿白细胞中的干扰素γ（IFN-γ）和吲哚胺 2,3-双加氧酶 1（IDO1）值，并将其与健康同龄对照组进行比较。
这是一项 2019-2020 年进行的前瞻性观察性病例对照研究。
意大利都灵 Regina Margherita 儿童医院。
该研究纳入了 63 名婴儿，包括 26 名因呼吸道合胞病毒（RSV）感染而住院的毛细支气管炎患儿和 37 名年龄匹配的对照组婴儿。纳入标准包括毛细支气管炎患儿 RSV 检测阳性。
我们采集外周血，采用 TaqMan 实时 PCR 扩增法测定 IFN-γ和 IDO1 的信使 RNA（mRNA）相对定量表达。数据采集于入院第 1 天。
26 例 RSV 毛细支气管炎患儿（53.8%为女性）的平均年龄为 85（9-346）天。他们的平均胎龄为 38 周，平均出生体重为 3100（2780-3730）g。与健康对照组相比，RSV 毛细支气管炎患儿 IFN-γ 的表达显著降低（p=0.0132）。然而，两组白细胞计数中 IDO1 mRNA 表达值无显著差异（p=0.0642）。
我们的研究结果并未阐明 IDO1 表达是否与疾病早期或婴儿年龄有关。数据表明，与年龄匹配的健康对照组相比，RSV 引起的毛细支气管炎患儿 IFN-γ 显著降低，但 IDO1 无差异。关注不同年龄段 RSV 感染患儿的新研究将有助于阐明 IDO1 表达是否与年龄有关。
