Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, 1400, Vienna, Austria.
Insectarium de Bobo Dioulasso-Campagne d'Eradication de la mouche tsetse et de la Trypanosomose (IBD-CETT), 01 BP 1087, Bobo Dioulasso 01, Burkina Faso.
Sci Rep. 2022 Feb 28;12(1):3322. doi: 10.1038/s41598-022-06699-2.
The sterile insect technique (SIT) is an environment friendly and sustainable method to manage insect pests of economic importance through successive releases of sterile irradiated males of the targeted species to a defined area. A mating of a sterile male with a virgin wild female will result in no offspring, and ultimately lead to the suppression or eradication of the targeted population. Tsetse flies, vectors of African Trypanosoma, have a highly regulated and defined microbial fauna composed of three bacterial symbionts that may have a role to play in the establishment of Trypanosoma infections in the flies and hence, may influence the vectorial competence of the released sterile males. Sodalis bacteria seem to interact with Trypanosoma infection in tsetse flies. Field-caught tsetse flies of ten different taxa and from 15 countries were screened using PCR to detect the presence of Sodalis and Trypanosoma species and analyse their interaction. The results indicate that the prevalence of Sodalis and Trypanosoma varied with country and tsetse species. Trypanosome prevalence was higher in east, central and southern African countries than in west African countries. Tsetse fly infection rates with Trypanosoma vivax and T. brucei sspp were higher in west African countries, whereas tsetse infection with T. congolense and T. simiae, T. simiae (tsavo) and T. godfreyi were higher in east, central and south African countries. Sodalis prevalence was high in Glossina morsitans morsitans and G. pallidipes but absent in G. tachinoides. Double and triple infections with Trypanosoma taxa and coinfection of Sodalis and Trypanosoma were rarely observed but it occurs in some taxa and locations. A significant Chi square value (< 0.05) seems to suggest that Sodalis and Trypanosoma infection correlate in G. palpalis gambiensis, G. pallidipes and G. medicorum. Trypanosoma infection seemed significantly associated with an increased density of Sodalis in wild G. m. morsitans and G. pallidipes flies, however, there was no significant impact of Sodalis infection on trypanosome density.
不育昆虫技术（SIT）是一种通过向特定区域连续释放经辐射处理的不育雄性目标物种来管理经济重要性昆虫害虫的环保且可持续的方法。不育雄性与野生处女雌性的交配不会产生后代，最终将导致目标种群的抑制或根除。采采蝇是非洲锥虫的传播媒介，其微生物群受到高度调节和定义，由三种细菌共生体组成，这些共生体可能在采采蝇中建立锥虫感染方面发挥作用，因此可能影响释放的不育雄性的媒介能力。Sodalis 细菌似乎与采采蝇中的锥虫感染相互作用。使用 PCR 对来自 15 个国家的 10 种不同分类的野外捕获的采采蝇进行筛选，以检测 Sodalis 和锥虫物种的存在，并分析它们的相互作用。结果表明，Sodalis 和锥虫的流行率因国家和采采蝇物种而异。东非、中非和南非国家的锥虫流行率高于西非国家。西非国家的采采蝇感染锥虫 vivax 和 T. brucei sspp 的比率较高，而东非、中非和南非国家的采采蝇感染 T. congolense 和 T. simiae、T. simiae（tsavo）和 T. godfreyi 的比率较高。G. morsitans morsitans 和 G. pallidipes 中 Sodalis 的流行率较高，但 G. tachinoides 中不存在。很少观察到锥虫分类群的双重和三重感染以及 Sodalis 和锥虫的合并感染，但在某些分类群和地点中确实存在。Sodalis 和 Trypanosoma 感染似乎存在显著相关性（<0.05），似乎表明在 G. palpalis gambiensis、G. pallidipes 和 G. medicorum 中存在这种相关性。锥虫感染似乎与野生 G. m. morsitans 和 G. pallidipes 采采蝇中 Sodalis 的密度增加显著相关，然而，Sodalis 感染对锥虫密度没有显著影响。
