Laboratory for Genome Engineering and Synthetic Biology, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
Plant Physiol. 2021 Aug 3;186(4):1770-1785. doi: 10.1093/plphys/kiab220.
Molecular engineering of plant immunity to confer resistance against plant viruses holds great promise for mitigating crop losses and improving plant productivity and yields, thereby enhancing food security. Several approaches have been employed to boost immunity in plants by interfering with the transmission or lifecycles of viruses. In this review, we discuss the successful application of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) (CRISPR/Cas) systems to engineer plant immunity, increase plant resistance to viruses, and develop viral diagnostic tools. Furthermore, we examine the use of plant viruses as delivery systems to engineer virus resistance in plants and provide insight into the limitations of current CRISPR/Cas approaches and the potential of newly discovered CRISPR/Cas systems to engineer better immunity and develop better diagnostics tools for plant viruses. Finally, we outline potential solutions to key challenges in the field to enable the practical use of these systems for crop protection and viral diagnostics.
通过分子工程手段来增强植物的免疫力以抵抗植物病毒，有望减轻作物损失，提高植物生产力和产量，从而增强粮食安全。人们已经采用了几种方法来通过干扰病毒的传播或生命周期来增强植物的免疫力。在这篇综述中，我们讨论了成功应用成簇规律间隔短回文重复序列（CRISPR）/CRISPR 相关蛋白（Cas）（CRISPR/Cas）系统来工程植物免疫、提高植物对病毒的抗性以及开发病毒诊断工具。此外，我们还研究了利用植物病毒作为载体来工程植物的病毒抗性，并深入探讨了当前 CRISPR/Cas 方法的局限性以及新发现的 CRISPR/Cas 系统在工程更好的免疫和开发更好的植物病毒诊断工具方面的潜力。最后，我们概述了该领域的关键挑战的潜在解决方案，以使这些系统能够实际用于作物保护和病毒诊断。
