Silva Fredy D A, Fontes Elizabeth P B
Department of Biochemistry and Molecular Biology/Bioagro, National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, Viçosa, Brazil.
Front Plant Sci. 2022 May 26;13:904829. doi: 10.3389/fpls.2022.904829. eCollection 2022.
Different genome editing approaches have been used to engineer resistance against plant viruses. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas; CRISPR/Cas) systems to create pinpoint genetic mutations have emerged as a powerful tool for molecular engineering of plant immunity and increasing resistance against plant viruses. This review presents (i) recent advances in engineering resistance against plant viruses by CRISPR/Cas and (ii) an overview of the potential host factors as targets for the CRISPR/Cas system-mediated broad-range resistance and immunity. Applications, challenges, and perspectives in enabling the CRISPR/Cas system for crop protection are also outlined.
不同的基因组编辑方法已被用于构建对植物病毒的抗性。用于产生精确基因突变的成簇规律间隔短回文重复序列（CRISPR）/CRISPR相关蛋白（Cas；CRISPR/Cas）系统，已成为植物免疫分子工程和增强对植物病毒抗性的有力工具。本综述介绍了（i）CRISPR/Cas介导的植物病毒抗性工程的最新进展，以及（ii）作为CRISPR/Cas系统介导的广谱抗性和免疫靶点的潜在宿主因子概述。还概述了CRISPR/Cas系统用于作物保护的应用、挑战和前景。
