College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, China.
College of Life Sciences, Langfang Normal University, Langfang, 065000, China.
Curr Microbiol. 2022 Mar 3;79(4):122. doi: 10.1007/s00284-022-02819-3.
Strain XMGL2, isolated from rhizosphere soil of Quercus mongolica in China, was characterized using a polyphasic taxonomic approach. Cells were Gram-negative, aerobic, non-spore-forming, and rod-shaped. Growth occurred at 20-37 °C (optimum, 28 °C), pH 5.0-10.0 (optimum, pH 6.0), and with 0-1% NaCl (optimum, 1%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XMGL2 was related to members of the genus Sphingomonas and had the highest 16S rRNA gene sequence identity to Sphingomonas oleivorans FW-11 (96.4%). The average nucleotide identity and digital DNA-DNA hybridization values between strain XMGL2 and the closely related taxa Sphingomonas oleivorans FW-11 and Sphingomonas fennica K101 were 75.3/19.8% and 75.8/20.2%, respectively. The major cellular fatty acids were C ω7c, C 2-OH, and C. The major isoprenoid quinone was Q-10 and the polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylmonomethylethanolamine, an unidentified glycophospholipid and an unidentified phospholipid. The genomic DNA G + C content was 67.9%. Based on the phenotypic and genotypic properties and phylogenetic inference, strain XMGL2 represents a novel species of the genus Sphingomonas, for which the name Sphingomonas quercus sp. nov. is proposed. The type strain is XMGL2 (= JCM 34441 = GDMCC 1.2153).
XMGL2 菌株是从中国蒙古栎根际土壤中分离出来的，采用多相分类方法进行了表征。细胞呈革兰氏阴性、需氧、非孢子形成、杆状。生长在 20-37°C（最佳温度 28°C），pH 值 5.0-10.0（最佳 pH 值 6.0），0-1%NaCl（最佳 1%）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 XMGL2 与鞘氨醇单胞菌属的成员有关，与 Sphingomonas oleivorans FW-11 的 16S rRNA 基因序列相似度最高（96.4%）。菌株 XMGL2 与密切相关的分类群 Sphingomonas oleivorans FW-11 和 Sphingomonas fennica K101 的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 75.3/19.8%和 75.8/20.2%。主要细胞脂肪酸为 C ω7c、C 2-OH 和 C。主要类异戊二烯醌为 Q-10，极性脂谱含有双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰二甲乙醇胺、磷脂单乙醇胺、一种未鉴定的糖脂和一种未鉴定的磷脂。基因组 DNA G+C 含量为 67.9%。基于表型和基因型特征以及系统发育推断，菌株 XMGL2 代表鞘氨醇单胞菌属的一个新种，提议命名为栎鞘氨醇单胞菌（Sphingomonas quercus sp. nov.）。模式菌株为 XMGL2（=JCM 34441=GDMCC 1.2153）。
