College of Biological Sciences and Technology, National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, 100083, China.
College of Life Sciences, Langfang Normal University, Langfang, 065000, China.
Antonie Van Leeuwenhoek. 2023 Aug;116(8):763-772. doi: 10.1007/s10482-023-01845-w. Epub 2023 May 24.
A Gram-stain-negative, rod-shaped, non-flagellated, pale-yellow bacterium, designated GHJ8, was isolated from the rhizosphere soil of Ulmus pumila L., Shanxi Province, China. Growth occurred at 20-37 °C (optimum, 28 °C), pH 6.0-11.0 (optimum, pH 8.0), and 0-1% NaCl (optimum, 0%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GHJ8 was related to members of the genus Luteolibacter, and close to Luteolibacter flavescens GKX (98.5%), Luteolibacter luteus G-1-1-1 (97.3%), Luteolibacter arcticus MC 3726 (97.2%), and Luteolibacter marinus NBU1238 (96.0%). The genome size of strain GHJ8 was 6.2 Mbp, with a G + C content of 62.5%. Genomic mining revealed that the strain contained antibiotic resistance genes and secondary metabolic gene clusters, indicating that it had adaptation mechanisms to environmental stress. Comparative genomic analyses clearly separated strain GHJ8 from the recognized species of the genus Luteolibacter based on average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values below the thresholds for species delineation. The major cellular fatty acids were iso-C (30.8%), C ω9c (23.0%), C (17.3%), and C (13.4%). The quinone system was composed of the major menaquinones MK-8, MK-9, and MK-10, and the principal polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified glycolipid, two unidentified phospholipids, and three unidentified lipids. Based on its phenotypic and genotypic properties and phylogenetic inference, strain GHJ8 is a novel species of the genus Luteolibacter, for which the name Luteolibacter rhizosphaerae sp. nov. is proposed. The type strain is GHJ8 (= GDMCC 1.2160 = KCTC 82452 = JCM 34400).
一株革兰氏阴性、杆状、无鞭毛、浅黄色的细菌，被命名为GHJ8，从中国山西省的榆树根际土壤中分离得到。生长在 20-37°C（最佳温度为 28°C）、pH 值为 6.0-11.0（最佳 pH 值为 8.0）和 0-1%NaCl（最佳 0%）的环境中。基于 16S rRNA 基因序列的系统发育分析表明，菌株 GHJ8 与 Luteolibacter 属的成员密切相关，与 Luteolibacter flavescens GKX（98.5%）、Luteolibacter luteus G-1-1-1（97.3%）、Luteolibacter arcticus MC 3726（97.2%）和 Luteolibacter marinus NBU1238（96.0%）最为接近。菌株 GHJ8 的基因组大小为 6.2 Mbp，G+C 含量为 62.5%。基因组挖掘显示，该菌株含有抗生素抗性基因和次级代谢基因簇，表明其具有适应环境压力的机制。比较基因组分析清楚地表明，基于平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值低于种系划分的阈值，菌株 GHJ8 与已识别的 Luteolibacter 属物种明显分离。主要的细胞脂肪酸为 iso-C（30.8%）、C ω9c（23.0%）、C（17.3%）和 C（13.4%）。醌系统由主要的menaquinones MK-8、MK-9 和 MK-10 组成，主要的极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基磷脂、一种未鉴定的糖脂、两种未鉴定的磷脂和三种未鉴定的脂类。基于其表型和基因型特征以及系统发育推断，菌株 GHJ8 是 Luteolibacter 属的一个新种，被命名为 Luteolibacter rhizosphaerae sp. nov.。模式菌株为 GHJ8（=GDMCC 1.2160=KCTC 82452=JCM 34400）。
