Banbury Carl, Styles Iain, Eisenstein Neil, Zanier Elisa R, Vegliante Gloria, Belli Antonio, Logan Ann, Goldberg Oppenheimer Pola
School of Chemical Engineering, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Computer Science, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Biomed Opt Express. 2020 Oct 8;11(11):6249-6261. doi: 10.1364/BOE.399473. eCollection 2020 Nov 1.
Traumatic brain injury (TBI) is a major burden on healthcare services worldwide, where scientific and clinical innovation is needed to provide better understanding of biochemical damage to improve both pre-hospital assessment and intensive care monitoring. Here, we present an unconventional concept of using Raman spectroscopy to measure the biochemical response to the retina in an murine model of TBI. Through comparison to spectra from the brain and retina following injury, we elicit subtle spectral changes through the use of multivariate analysis, linked to a decrease in cardiolipin and indicating metabolic disruption. The ability to classify injury severity via spectra of the retina is demonstrated for severe TBI (82.0 %), moderate TBI (75.1 %) and sham groups (69.4 %). By showing that optical spectroscopy can be used to explore the eye as the window to the brain, we lay the groundwork for further exploitation of Raman spectroscopy for indirect, non-invasive assessment of brain chemistry.
创伤性脑损伤（TBI）是全球医疗服务的一项重大负担，需要科学和临床创新来更好地理解生化损伤，以改善院前评估和重症监护监测。在此，我们提出了一个非传统的概念，即在创伤性脑损伤的小鼠模型中使用拉曼光谱来测量视网膜的生化反应。通过与损伤后脑和视网膜的光谱进行比较，我们利用多变量分析得出了细微的光谱变化，这与心磷脂的减少有关，并表明存在代谢紊乱。对于重度创伤性脑损伤组（82.0%）、中度创伤性脑损伤组（75.1%）和假手术组（69.4%），均证明了通过视网膜光谱对损伤严重程度进行分类的能力。通过表明光谱学可用于将眼睛作为大脑的窗口进行探索，我们为进一步利用拉曼光谱对脑化学进行间接、非侵入性评估奠定了基础。
