迈向千克级过氧酶催化的环己烷氧官能化反应
Toward Kilogram-Scale Peroxygenase-Catalyzed Oxyfunctionalization of Cyclohexane.
作者信息
Hilberath Thomas, van Oosten Remco, Victoria Juliet, Brasselet Hugo, Alcalde Miguel, Woodley John M, Hollmann Frank
机构信息
Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629HZ Delft, The Netherlands.
Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
出版信息
Org Process Res Dev. 2023 Jun 9;27(7):1384-1389. doi: 10.1021/acs.oprd.3c00135. eCollection 2023 Jul 21.
Mol-scale oxyfunctionalization of cyclohexane to cyclohexanol/cyclohexanone (KA-oil) using an unspecific peroxygenase is reported. Using UPO from and simple HO as an oxidant, cyclohexanol concentrations of more than 300 mM (>60% yield) at attractive productivities (157 mM h, approx. 15 g L h) were achieved. Current limitations of the proposed biooxidation system have been identified paving the way for future improvements and implementation.
报道了使用非特异性过氧化物酶将环己烷分子尺度氧官能化为环己醇/环己酮（KA油）的过程。使用来自[具体来源未给出]的非特异性过氧化物酶（UPO）和简单的过氧化氢（HO）作为氧化剂，在具有吸引力的生产率（157 mM/h，约15 g/L/h）下实现了超过300 mM的环己醇浓度（产率>60%）。已确定了所提出的生物氧化系统当前的局限性，为未来的改进和实施铺平了道路。
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