Huovinen P, Pulkkinen L, Helin H L, Mäkilä M, Toivanen P
Antimicrob Agents Chemother. 1986 Jan;29(1):73-6. doi: 10.1128/AAC.29.1.73.
Emergence of trimethoprim resistance among urinary tract Escherichia coli strains, isolated mostly from long-term patients in the Turku City Hospital, Turku, Finland, was studied from 1971 through 1984. Emergence of resistance to trimethoprim was associated with changes in the consumption of both trimethoprim-sulfamethoxazole and trimethoprim, with occurrence of high-level trimethoprim resistance and sequences homologous to trimethoprim resistance transposon Tn7. Since 1971, resistance of E. coli to trimethoprim-sulfamethoxazole increased from 8 to between 32 and 35% in 1983 and 1984; resistance to sulfamethoxazole varied from 39 to between 40 and 44%. The frequency of DNA sequence homology with our Tn7 probe among trimethoprim-resistant E. coli strains was 42% from 1980 to 1981 and 64% in 1983 (P less than 0.005). Fourteen years after the introduction of trimethoprim therapy in this hospital, resistance has reached the level of resistance to sulfonamide.
对1971年至1984年间从芬兰图尔库市立医院的长期患者中分离出的尿路大肠杆菌菌株对甲氧苄啶耐药性的出现情况进行了研究。对甲氧苄啶耐药性的出现与甲氧苄啶-磺胺甲恶唑和甲氧苄啶的使用变化、高水平甲氧苄啶耐药性的出现以及与甲氧苄啶耐药转座子Tn7同源的序列有关。自1971年以来，大肠杆菌对甲氧苄啶-磺胺甲恶唑的耐药性从8%增加到1983年和1984年的32%至35%之间；对磺胺甲恶唑的耐药性从39%变化到40%至44%之间。1980年至1981年间，耐甲氧苄啶大肠杆菌菌株中与我们的Tn7探针DNA序列同源性频率为42%，1983年为64%（P小于0.005）。在该医院引入甲氧苄啶治疗14年后，耐药性已达到对磺胺类药物的耐药水平。
