Whelan W L, Markie D, Kwon-Chung K J
Antimicrob Agents Chemother. 1986 May;29(5):726-9. doi: 10.1128/AAC.29.5.726.
A complementation test was devised to study allelism among the genetic determinants of resistance to 5-fluorocytosine in Candida albicans. Complementation was demonstrated in control hybrids produced by crossing a resistant strain that was deficient in cytosine deaminase activity with four other resistant strains deficient in UMP pyrophosphorylase activity. This complementation test was used to test allelism of the resistance determinants present in five clinical isolates. All were found to bear recessive alleles of the locus (FCY1) that determined 5-fluorocytosine resistance associated with low levels of UMP pyrophosphorylase activity.
设计了一项互补试验来研究白色念珠菌中对5-氟胞嘧啶抗性的遗传决定因素之间的等位性。通过将一株胞嘧啶脱氨酶活性缺陷的抗性菌株与另外四株UMP焦磷酸化酶活性缺陷的抗性菌株杂交产生的对照杂种中证实了互补作用。该互补试验用于测试五个临床分离株中存在的抗性决定因素的等位性。发现所有分离株都携带该位点（FCY1）的隐性等位基因，该基因决定了与低水平UMP焦磷酸化酶活性相关的5-氟胞嘧啶抗性。
