Goshorn A K, Scherer S
Department of Microbiology, University of Minnesota School of Medicine, Minneapolis 55455.
Genetics. 1989 Dec;123(4):667-73. doi: 10.1093/genetics/123.4.667.
To facilitate genetic analysis of Candida albicans natural variants, we have isolated a dominant mycophenolic acid-resistant mutant. Mycophenolic acid-resistant auxotrophs were used to analyze prototrophic natural variants by spheroplast fusion. The fusion products were shown to be heterozygous for many of the parental chromosomes by molecular and genetic criteria. Using this approach, we have found that one type of morphologic variation is due to a recessive change and identified three dominant 5-fluorocytosine-resistant mutants. Rare fusion products express recessive parental markers. These exceptional progeny should be useful for linkage analysis and strain construction.
为便于对白色念珠菌自然变体进行遗传分析，我们分离出了一株显性霉酚酸抗性突变体。利用对霉酚酸具有抗性的营养缺陷型菌株，通过原生质体融合来分析原养型自然变体。通过分子和遗传学标准表明，融合产物在许多亲代染色体上是杂合的。利用这种方法，我们发现一种形态变异类型是由隐性变化引起的，并鉴定出三个显性5-氟胞嘧啶抗性突变体。罕见的融合产物表达隐性亲代标记。这些特殊的子代应有助于连锁分析和菌株构建。
