Institute of Bioadditive Manufacturing, Jiangxi University of Science and Technology, Nanchang 330013, People's Republic of China.
Institute of Innovation and Applied Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, People's Republic of China.
Nanotechnology. 2022 Mar 25;33(24). doi: 10.1088/1361-6528/ac5aee.
Black phosphorus (BP) exhibits great potential as antibacterial materials due to its unique photocatalytic activity. However, the unsatisfactory optical absorption and quick recombination of photoinduced electron-hole pairs restrain its photocatalytic antibacterial performance. In this work, silver nanoparticles (AgNPs) were decorated on BP to construct BP@AgNPs nanohybrids and then introduced into poly-l-lactic acid scaffold. Combining the tunable bandgap of BP and the LSPR effect of AgNPs, BP@AgNPs nanohybrids displayed the broaden visible light absorption. Furthermore, AgNPs acted as electron acceptors could accelerate charge transfer and suppress electron-hole recombination. Therefore, BP@AgNPs nanohybrids achieved synergistically enhanced photocatalytic antibacterial activity under visible light irradiation. Fluorescence probe experiment verified that BP@AgNPs promoted the generation of reactive oxygen species, which could disrupt bacteria membrane, damage DNA and oxide proteins, and finally lead to bacteria apoptosis. As a result, the scaffold possessed strong antibacterial efficiency with a bactericidal rate of 97% under light irradiation. Moreover, the scaffold also exhibited good cytocompatibility. This work highlighted a new strategy to develop photocatalytic antibacterial scaffold for bone implant application.
黑磷（BP）由于其独特的光催化活性，在作为抗菌材料方面表现出巨大的潜力。然而，光生电子-空穴对的光吸收不理想和快速复合限制了其光催化抗菌性能。在这项工作中，银纳米粒子（AgNPs）被修饰在 BP 上，构建了 BP@AgNPs 纳米杂化材料，然后将其引入聚-l-乳酸支架中。结合 BP 的可调带隙和 AgNPs 的局域表面等离子体共振效应，BP@AgNPs 纳米杂化材料表现出了宽的可见光吸收。此外，AgNPs 作为电子受体可以加速电荷转移并抑制电子-空穴复合。因此，BP@AgNPs 纳米杂化材料在可见光照射下协同增强了光催化抗菌活性。荧光探针实验验证了 BP@AgNPs 促进了活性氧的生成，活性氧可以破坏细菌膜、损伤 DNA 和氧化蛋白质，最终导致细菌凋亡。结果，在光照下，支架具有很强的抗菌效率，杀菌率达到 97%。此外，支架还表现出良好的细胞相容性。这项工作为开发用于骨植入应用的光催化抗菌支架提供了一种新策略。
