Shuai Cijun, Chen Xuan, He Chongxian, Qian Guowen, Shuai Yang, Peng Shuping, Deng Youwen, Yang Wenjing
Institute of Additive Manufacturing, Jiangxi University of Science and Technology, Nanchang, 330013, China.
State Key Laboratory of High Performance Complex Manufacturing, Central South University, Changsha, 410083, China.
Biomater Res. 2022 Aug 6;26(1):38. doi: 10.1186/s40824-022-00278-2.
FeO nanoparticles are highly desired for constructing endogenous magnetic microenvironment in scaffold to accelerate bone regeneration due to their superior magnetism. However, their random arrangement easily leads to mutual consumption of magnetic poles, thereby weakening the magnetic stimulation effect.
In this study, magnetic nanochains are synthesized by magnetic-field-guided interface co-assembly of FeO nanoparticles. In detail, multiple FeO nanoparticles are aligned along the direction of magnetic force lines and are connected in series to form nanochain structures under an external magnetic field. Subsequently, the nanochain structures are covered and fixed by depositing a thin layer of silica (SiO), and consequently forming linear magnetic nanochains (FeO@SiO). The FeO@SiO nanochains are then incorporated into poly l-lactic acid (PLLA) scaffold prepared by selective laser sintering technology.
The results show that the FeO@SiO nanochains with unique core-shell structure are successfully constructed. Meanwhile, the orderly assembly of nanoparticles in the FeO@SiO nanochains enable to form magnetic energy coupling and obtain a highly magnetic micro-field. The in vitro tests indicate that the PLLA/FeO@SiO scaffolds exhibit superior capacity in enhancing cell activity, improving osteogenesis-related gene expressions, and inducing cell mineralization compared with PLLA and PLLA/FeO scaffolds.
In short, the FeO@SiO nanochains endow scaffolds with good magnetism and cytocompatibility, which have great potential in accelerating bone repair.
由于其优异的磁性，FeO纳米颗粒在构建支架内源性磁微环境以加速骨再生方面备受青睐。然而，它们的随机排列容易导致磁极相互消耗，从而削弱磁刺激效果。
在本研究中，通过FeO纳米颗粒的磁场引导界面共组装合成磁性纳米链。具体而言，多个FeO纳米颗粒沿磁力线方向排列，并在外部磁场作用下串联连接形成纳米链结构。随后，通过沉积一层二氧化硅（SiO）覆盖并固定纳米链结构，从而形成线性磁性纳米链（FeO@SiO）。然后将FeO@SiO纳米链掺入通过选择性激光烧结技术制备的聚L-乳酸（PLLA）支架中。
结果表明，成功构建了具有独特核壳结构的FeO@SiO纳米链。同时，FeO@SiO纳米链中纳米颗粒的有序组装能够形成磁能耦合并获得高磁场微环境。体外测试表明，与PLLA和PLLA/FeO支架相比，PLLA/FeO@SiO支架在增强细胞活性、改善成骨相关基因表达和诱导细胞矿化方面表现出优异的能力。
简而言之，FeO@SiO纳米链赋予支架良好的磁性和细胞相容性，在加速骨修复方面具有巨大潜力。
