Research and Development Department, ROXALL Medicina España S.A., Zamudio, Spain.
Research and Development Department, ROXALL Medicina España S.A., Zamudio, Spain.
Ann Allergy Asthma Immunol. 2022 Jul;129(1):101-105. doi: 10.1016/j.anai.2022.02.018. Epub 2022 Mar 2.
Vespa velutina nigrithorax (VVN), typically known as the Asian yellow-legged wasp, has been one of the most significant invasive species in western Europe since 2010. Currently, VVN has become the most prevalent cause of Hymenoptera anaphylaxis in the north and northwestern Spain. For this reason, it is crucial to diagnose anaphylaxis cases in the acute moment for carrying out the best available treatment as soon as possible.
To achieve a complete understanding of the venom allergen composition that will help to develop efficient diagnostics and immunotherapy treatments on the basis of this venom.
In this study, autochthonous VVN venom was obtained and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, isoelectric focusing, followed by a mass spectrometry analysis. In addition, the allergenic sensitization profile of patients diagnosed with allergy to VVN in the Allergology Service of Navarra University Hospital between the years 2017 and 2020 was studied by immunoblotting and specific IgE (ImmunoCAP, Thermo Fisher Scientific, Uppsala, Sweden).
Two new allergens (dipeptidyl peptidase IV and serin protease) were identified in the autochthonous VVN venom, and their identity was confirmed by liquid chromatography-mass spectrometry analysis. The study by ImmunoCAP using sera from 12 patients who had a systemic reaction after a VVN sting revealed groups 5 and 1 as predominant allergens (92% and 34%, respectively). Furthermore, the immunoblotting assay recognized dipeptidyl peptidase IV (50%) in the sera of these patients.
Serine protease and the dipeptidyl peptidase IV are components of the VVN venom, and the latter is an allergen recognized in the studied population.
亚洲大黄蜂（VVN）通常被称为亚洲黄脚胡蜂，自 2010 年以来一直是西欧最重要的入侵物种之一。目前，VVN 已成为西班牙北部和西北部最常见的蜂类过敏反应原因。因此，在急性时刻诊断过敏反应病例并尽快进行最佳治疗至关重要。
全面了解毒液过敏原组成，有助于在此毒液基础上开发有效的诊断和免疫治疗方法。
在这项研究中，我们获得了本土 VVN 毒液，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、等电聚焦和质谱分析对其进行了表征。此外，我们还通过免疫印迹和特异性 IgE（ImmunoCAP，Thermo Fisher Scientific，Uppsala，瑞典）分析了 2017 年至 2020 年期间在纳瓦拉大学医院过敏科诊断为 VVN 过敏的患者的致敏谱。
在本土 VVN 毒液中鉴定出两种新的过敏原（二肽基肽酶 IV 和丝氨酸蛋白酶），并通过液相色谱-质谱分析确认了它们的身份。使用 12 例 VVN 蜇伤后发生全身性反应的患者的血清进行 ImmunoCAP 研究显示，组 5 和组 1 是主要过敏原（分别为 92%和 34%）。此外，免疫印迹检测到这些患者血清中的二肽基肽酶 IV（50%）。
丝氨酸蛋白酶和二肽基肽酶 IV 是 VVN 毒液的成分，后者是研究人群中识别的过敏原。
