Brown J C, Harris G, Papamichail M, Sljivić V S, Holborow E J
Immunology. 1973 Jun;24(6):955-68.
The localization of aggregated human γ-globulin in the germinal centres of the white pulp of the spleens of normal mice was found to be dependent on the ability of the spleen to concentrate this material from the blood and of lymphoid cells in the mantle layer of the Malpighian bodies to take it up on their surface membranes. The fact that lymphoid cells resident in the spleen perform this function was shown by the inability of lymphoid cells transferred to recipient animals to transport the aggregated material to the spleens of the latter. Ionizing radiation in relatively high doses prevented the localization of aggregated human γ-globulin and was effective only when given before or less than 6 hours after administration of the aggregated material. Spleens shielded during whole body irradiation were capable of localizing the material in germinal centres, but local irradiation of the spleens with protection of the rest of the body prevented splenic localization. These results support the conclusion that localization of altered heterologous antibody is a function of lymphoid cells already present in the spleens at the time of injection of this material and is not dependent on its transport into the white pulp by migrating cells which pick it up in the blood or elsewhere. Antilymphocytic serum also prevented localization of aggregated human γ-globulin, suggesting a direct effect of this antiserum on non-circulating lymphoid cells. However, the manner of its action requires further study.
已发现，聚集的人γ球蛋白在正常小鼠脾脏白髓生发中心的定位，取决于脾脏从血液中浓缩这种物质的能力，以及马尔皮基小体被膜层中淋巴细胞在其表面膜上摄取它的能力。驻留在脾脏中的淋巴细胞执行此功能这一事实，由转移至受体动物的淋巴细胞无法将聚集物质转运至后者的脾脏得以证明。相对高剂量的电离辐射可阻止聚集的人γ球蛋白的定位，且仅在给予聚集物质之前或之后不到6小时给予时才有效。全身照射期间受到屏蔽的脾脏能够将物质定位于生发中心，但对脾脏进行局部照射并对身体其他部位加以保护则会阻止脾脏定位。这些结果支持以下结论：改变的异源抗体的定位是在注射该物质时已存在于脾脏中的淋巴细胞的功能，并不依赖于通过在血液或其他部位摄取它的游走细胞将其转运至白髓。抗淋巴细胞血清也可阻止聚集的人γ球蛋白的定位，提示这种抗血清对非循环淋巴细胞有直接作用。然而，其作用方式尚需进一步研究。
