The localization of aggregated human -globulin in the spleens of normal mice.

The localization of aggregated human γ-globulin in the germinal centres of the white pulp of the spleens of normal mice was found to be dependent on the ability of the spleen to concentrate this material from the blood and of lymphoid cells in the mantle layer of the Malpighian bodies to take it up on their surface membranes. The fact that lymphoid cells resident in the spleen perform this function was shown by the inability of lymphoid cells transferred to recipient animals to transport the aggregated material to the spleens of the latter. Ionizing radiation in relatively high doses prevented the localization of aggregated human γ-globulin and was effective only when given before or less than 6 hours after administration of the aggregated material. Spleens shielded during whole body irradiation were capable of localizing the material in germinal centres, but local irradiation of the spleens with protection of the rest of the body prevented splenic localization. These results support the conclusion that localization of altered heterologous antibody is a function of lymphoid cells already present in the spleens at the time of injection of this material and is not dependent on its transport into the white pulp by migrating cells which pick it up in the blood or elsewhere. Antilymphocytic serum also prevented localization of aggregated human γ-globulin, suggesting a direct effect of this antiserum on non-circulating lymphoid cells. However, the manner of its action requires further study.