Chambers R, Groufsky A, Hunt J S, Lynn K L, McGiven A R
Clin Nephrol. 1986 Jul;26(1):21-6.
Tamm-Horsfall glycoprotein (TH) distribution was studied using a biotin-avidin immunoperoxidase technique in renal biopsies from 166 consecutive patients and 8 normal kidneys. Tubulointerstitial damage was independently assessed and graded. In 109 patients TH antibodies were measured by ELISA and in 30 of these urinary TH and beta 2-microglobulin excretions were measured by radioimmunoassay. In 124 biopsies only distal tubular epithelium and casts were stained. Glomerular space (8) or interstitial (34) deposits were seen in 42 biopsies; 16/68 with glomerulonephritis, 4/14 with systemic vasculitis, 12/33 with chronic interstitial nephritis, 1/8 with acute interstitial nephritis, 9/43 with other nephropathies. There was no correlation between TH distribution and the degree of tubulointerstitial damage (p greater than 0.5), urinary TH excretion (p greater than 0.05), urinary beta 2-microglobulin excretion (p greater than 0.05), glomerular filtration rate, urinary concentrating ability, or the incidence of pyuria. TH antibodies did not correlate with TH distribution (p greater than 0.5) or the degree of tubulointerstitial damage. Abnormal TH distribution showed no statistical relationship to the degree of tubulointerstitial damage, changes in renal function or levels of TH antibodies.
采用生物素-抗生物素蛋白免疫过氧化物酶技术，对166例连续患者的肾活检组织和8个正常肾脏进行了Tamm-Horsfall糖蛋白（TH）分布研究。对肾小管间质损伤进行了独立评估和分级。109例患者采用酶联免疫吸附测定法检测TH抗体，其中30例采用放射免疫测定法检测尿TH和β2-微球蛋白排泄量。124例活检标本中仅远端肾小管上皮和管型染色。42例活检标本可见肾小球间隙（8例）或间质（34例）沉积物；肾小球肾炎患者68例中有16例，系统性血管炎患者14例中有4例，慢性间质性肾炎患者33例中有12例，急性间质性肾炎患者8例中有1例，其他肾病患者43例中有9例。TH分布与肾小管间质损伤程度（p>0.5）、尿TH排泄量（p>0.05）、尿β2-微球蛋白排泄量（p>0.05）、肾小球滤过率、尿浓缩能力或脓尿发生率之间无相关性。TH抗体与TH分布（p>0.5）或肾小管间质损伤程度无关。TH分布异常与肾小管间质损伤程度肾功能变化或TH抗体水平无统计学关系。
